No abstract

Prevention of secondary infection is currently the main goal of treatment for acute necrotizing pancreatitis. Colon was considered as the main origin of secondary infection. Our aim was to investigate whether prophylactic total colectomy would reduce the rate of bacterial translocation and infection of pancreatic necrosis. Forty-two Sprague-Dawley rats were used. Pancreatitis was created by ductal infusion of sodium taurocholate. Rats were divided into four groups: group-1, laparotomy + pancreatic ductal infusion of saline; group-2, laparotomy + pancreatic ductal infusion of sodium taurocholate; group-3, total colectomy + pancreatic ductal infusion of saline; and group-4, total colectomy + pancreatic ductal infusion of sodium taurocholate. Forty-eight hours later, tissue and blood samples were collected for microbiological and histopathological analysis. Total colectomy caused small bowel bacterial overgrowth with gram-negative and gram-positive microorganisms. Bacterial count of gram-negative rods in the small intestine and pancreatic tissue in rats with colectomy and acute pancreatitis were significantly higher than in rats with acute pancreatitis only (group-2 versus group-4; small bowel, p = <0.001; pancreas, p = 0.002). Significant correlation was found between proximal small bowel bacterial overgrowth and pancreatic infection (r = 0,836, p = 0.001). In acute pancreatitis, prophylactic total colectomy (which can mimic colonic cleansing and reduction of colonic flora) induces small bowel bacterial overgrowth, which is associated with increased bacterial translocation to the pancreas.

BACKGROUND: Despite the development of clinical, laboratory, and imaging methods, the diagnosis of acute appendicitis is not always easy, and negative appendectomy rates are still high. This study aims to reveal the effects of different scoring systems on the diagnostic accuracy of acute appendicitis and negative appendectomy rates, alone or when evaluated together with ultrasonography. METHODS: In this study, 202 consecutive patients who underwent emergency appendectomy for acute appendicitis were included. Clinical scores of all patients were preoperatively calculated using Ohmann, Raja Isteri Pengiran Anak Saleha Appendicitis (RIPASA), Lintula, Eskelinen, and Alvarado scoring systems. Abdominal ultrasonography (USG) was performed randomly in all cases. The sensitivity and specificity of scoring systems were calculated according to the threshold values. The area under the curve (AUC) was calculated using ROC analysis. In the regression model, histological diagnosis of appendicitis was used as the dependent variable, while scoring systems and USG were preferred as independent variables. RESULTS: The negative appendectomy rate was 15.8%. In the diagnosis of acute appendicitis, Ohmann was the most predictive for both genders (DOR=24.2, 95% CI 6.98-84.44). Similarly, the lowest negative appendectomy rates were obtained with the Ohmann score as 6.9% in females and 3.4% in males. When the scores were combined with USG, the rate of diagnostic accuracy for acute appendicitis was not increased. However, when Ohmann and USG were combined, negative appendectomy rates were further reduced for women from 6.9% to 4%. CONCLUSION: In addition to being a good diagnostic predictor of acute appendicitis in male and female patients, Ohmann score provides the best negative appendectomy rates. The combination of USG and scoring systems does not increase the diagnostic accuracy of acute appendicitis. However, negative appendectomy rates are significantly reduced when the USG and Ohmann scale are used together in females, while this reduction is minimal in men.

OBJECTIVES: We aimed to investigate the effects of infl iximab and HBO (hyperbaric oxygen) used alone or in combination on oxidative stress and the severity of pancreatitis in an experimental model of AP (acute pancreatitis). MATERIAL AND METHODS: A total of 60 rats were randomly divided into fi ve groups. Group 1 underwent laparotomy; Group 2 underwent experimental AP; Group 3 was given an infl iximab infusion and underwent AP; Group 4 was subjected to HBO therapy after AP; and Group 5 was given infl iximab infusion before AP and subjected to HBO therapy. Serum amylase, superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GPX) levels in the pancreas tissues were measured. The pancreatic tissue samples were scored. RESULTS: There were statistically signifi cant differences in the histopathological scores and amylase levels between non-treated AP and all the three treatment groups. Group 5 had the closest histopathological scores to the sham group. MDA levels were signifi cantly different between non-treated AP and all the three treatment groups, but the SOD levels and GPX values were not. CONCLUSIONS: Combination of HBO therapy and Infl iximab showed a synergistic effect on the reduction of histopathological severity and mortality in acute pancreatitis. All treatment modalities reduced the pathological fi ndings by decreasing lipid peroxidation and partly increasing the antioxidant capacity in early period (Tab.

Çalışmanın amacı gebe kadınlarda görülen apandisitin tanı ve tedavi stratejileri ile klinikopatolojik özellikleri ve feto-maternal sonuçlarını değerlendirmektir. Gereç ve Yöntem: Bu çalışmada apendektomi yapılan 17 gebe kadın ve yaşları eşleştirilmiş 59 gebe olmayan kadın laboratuvar bulguları, preoperatif ultrasonografi (USG), patoloji ve klinik sonuçlar açısından karşılaştırıldı. Bulgular: Toplam USG tarama sayısı, görüntülenemeyen apandis oranları ve hastanede kalış süreleri gebe kadınlarda gebe olmayan kadınlara göre daha fazla idi (sırasıyla p<0,001, p=0,035 ve p=0,014). Gebe grupta negatif apendektomi oranı gebe olmayanlara göre 1,5 kat, komplike apandisit oranı ise 7 kat daha yüksekti. USG'nin tanısal doğruluğu ise gebe olmayan grupta daha yüksek bulundu (%72,9'a karşı %64,7). Hematolojik parametreler açısından apandisit olan ve olmayan gebe hastalar arasında anlamlı bir fark bulunmadı. İkinci trimesterde bir erken doğum ve bir abortus görülürken, üçüncü trimesterde bir hastada negatif apendektomiyi takiben bir erken doğum gerçekleşti. Sonuç: Gebelik sırasında laboratuvar parametreleri ve USG ile konulan apandisit tanısı hatalı olabilmektedir. Bu yüzden, gereksiz cerrahi müdahalelerden kaçınmak için, klinisyenlerin hamilelik sırasında apandisitten şüphelendikleri durumlarda ek görüntüleme tetkikleri yapmayı düşünmelerini öneririz. Çünkü hem negatif apendektomi hem de komplike apandisit, ihmal edilemez bir fetal morbidite ve mortalite oranıyla sonuçlanabilir.