The development of vanilla seeds through tissue culture techniques with the addition of growth regulator kinetin aims to increase the success of shoot formation and growth of vanilla explants. The research was carried out from February to June 2021 at the Tissue Culture Laboratory Politeknik Negeri Jember. The research method used a single factor Completely Randomized Design (CRD) with 4 treatments which were repeated 5 times. The treatment was the addition of kinetin to Murashige-Skoog (MS) media with a concentration of 0, 1, 2, 3 mg/L. Data analysis used analysis of variance followed by a 5% or 1% BNT test. The results of the analysis showed that the addition of kinetin affected the initial germination time of vanilla explants with the fastest time being 13-17 days after inoculation at a kinetin concentration of 1-3 mg/L. The kinetin concentration of 3 mg/L also affected the wet weight of vanilla explants aged 8 weeks after inoculation with the highest average of 2.62 grams per explant. However, the addition of kinetin did not affect root length, root number and shoot length until the vanilla explants were 8 weeks old after inoculation. .
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.