− Endothelial dysfunction in atherosclerosis is associated with increasing oxidative stress that could be reversed by antioxidant. Therefore epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and catechin (C) of tea flavonoids were investigated for their roles in regenerating endothelial cell. Peripheral blood mononuclear cells (PB-MNCs) were isolated, plated and cultured in medium with/without treatment of EGCG, ECG, EGC and C. Results showed that among all EGCG, ECG, EGC and C concentrations tested, 12.5 µmol/L was not cytotoxic for peripheral blood-derived endothelial progenitor cells (PB-EPCs). Treatment of EGCG, ECG, EGC or C increased the percentages of CD34, CD133, VEGFR-2 expressions and suppressed hydrogen peroxide-induced percentages of reactive oxygen species (ROS) level in PB-EPCs. Taken together, our current results showed that EGCG, ECG, EGC or C of tea flavonoids could induce differentiation of PB-MNCs into PB-EPCs as well as protect PB-EPCs from oxidative damage by suppresing the intracellular ROS levels.
<p align="center"><strong>ABSTRAK</strong></p><p><strong><em>Latar belakang</em></strong><em>: Kanker serviks merupakan penyakit yang menduduki posisi kedua penyebab kematian pada wanita. Berbagai terapi pendukung mulai dikembangkan, seperti melalui bahan makanan yang dipercaya memiliki efek anti</em><em>-</em><em>kanker. Buah sirsak disebut memiliki kandungan fitokimia seperti Annonaceous acetogenin, flavonoid dan fenol yang bermanfaat sebagai anti</em><em>-</em><em>kanker. </em></p><p><strong><em>Tujuan:</em></strong><em> Penelitian ini dilakukan untuk mengetahui potensi anti</em><em>-</em><em>oksidan dan sitotoksisitas dari sari buah sirsak pada sel HeLa secara in vitro. </em></p><p><strong><em>Metode</em></strong><em>: Penelitian ini dilakukan dengan membuat sari buah sirsak dengan cara diblender kemudian dikeringkan dengan metode freeze-drying, yang kemudian dilanjutkan dengan uji 2,2-diphenyl-1-picrylhydrazyl (DPPH) untuk mengetahui potensi penghambatan radikal bebas, dan potensi sitotoksisitas melalui uji MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difenitetrazolium bromide) assay pada sel kanker serviks HeLa. </em></p><p><strong><em>Hasil</em></strong><em>: Hasil uji aktivitas anti</em><em>-</em><em>oksidan menunjukkan persamaan regresi linier (y=0,0149x – 2,8812) dan nilai</em><em> perhitungan</em><em> IC<sub>50</sub> sari buah sirsak sebesar 3549 μg/mL<strong> </strong>dan hasil uji sitotoksisitas menunjukkan persamaan regresi linier (y=0,0197x + 0,3101) dan nilai</em><em> perhitungan</em><em> IC<sub>50</sub> sari buah sirsak pada sel HeLa sebesar 2522,33 μg/mL.</em></p><p><strong><em>Kesimpulan</em></strong><em>: Sari buah sirsak memiliki aktivitas anti</em><em>-</em><em>oksidan yang sangat rendah dan tidak berpotensi sebagai anti</em><em>-</em><em>kanker terhadap sel HeLa secara in vitro.</em><em></em></p><p> </p><p><strong>KATA KUNCI </strong><strong>:</strong><em> Annona muricata Linn, anti</em><em>-</em><em>oksidan, kanker, sitotoksisitas</em></p><p align="center"><strong> </strong></p><p align="center"><strong>ABSTRACT</strong></p><p><strong><em>Background: </em></strong><em>Cervical cancer is the second leading cause of death in women. Various supporting therapies have been developed, such as through food ingredients which are believed to have anti</em><em>-</em><em>cancer effects. Soursop </em><em>is known to be high</em><em> phytoc</em><em>h</em><em>emical content such as Annonaceous acetogenin, flavonoid and phenols which are useful as anti</em><em>-</em><em>cancer. </em></p><p><strong><em>Objectives:</em></strong><em> This research was conducted to determine the antioxidant and cytotoxic activity of soursop juice on HeLa cell lines.</em></p><p><strong><em>Methods: </em></strong><em>This study started</em><em> by</em><em> making the soursop fruit extract by blending then dried with freeze</em><em>-</em><em>drying method, and then proceed with 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay to determine the potential free radical scavenging activity, cytotoxic potential activity in vitro through MTT (3-(4,5-dimethylthiazol-2-il)-2,5-difenitetrazolium bromide) assay on HeLa cell lines.</em></p><p><strong><em>Results: </em></strong><em>The IC<sub>50</sub> antioxidant activity of soursop fruit extract is 3549 μg/mL with linear regression equation (y=0.0149x – 2.8812) and the IC<sub>50</sub> cytotoxicity test of soursop fruit extracton HeLa was 2522,33 μg/mL with linier regression equation (y=0.0197x + 0.3101).<strong> </strong></em></p><p><strong><em>Conclusion: </em></strong><em>The conclusion in this study is that soursop fruit extract has very low antioxidant activity and has no in vitro potential effect as an anti</em><em>-</em><em>cancer on HeLa cell lines.</em></p><p><em> </em></p><p><strong>KEYWORDS </strong><strong>:</strong><em> Annona muricata Linn, antioxidant, cancer, cytotoxic</em></p><p><em> </em></p>
Jenis kanker yang paling umum ditemui di rumah sakit di Indonesia adalah kanker payudara (28.7%) disusul kanker serviks (12.8%). Salah satu upaya terapi pendukung untuk kanker adalah melalui konsumsi buah-buahan. Buah Nanas (Ananas comosus) banyak diteliti potensinya sebagai antikanker. Buah nanas merupakan sumber antioksidan dan berfungsi sebagai agen antikanker lewat mekanisme aktivasi apoptosis sel kanker serta penghambatan aktivitas reseptor tirosin kinase. Tujuan penelitian ini untuk mengamati aktivitas antioksidan dan sitotoksisitas ekstrak metanol buah nanas terhadap sel T-47D secara In Vitro. Proses ekstraksi daging buah nanas dilakukan dengan memakai pelarut metanol, selanjutnya diuji aktivitas antioksidan dengan DPPH dan sitotoksisitas menggunakan uji MTT pada sel kanker payudara T-47D. Nilai IC50 aktivitas antioksidan menunjukkan hasil sebesar 1549.88 μg/ml dan IC50 untuk uji sitotoksisitas sebesar 741.46 μg/ml. Hasil penelitian menunjukkan ekstrak metanol buah nanas mempunyai aktivitas antioksidan yang sangat lemah dan sitotoksisitas moderat terhadap sel T-47D secara in vitro sehingga bisa sebagai agen kemopreventif.
Background: Breast cancer is the most common type of cancer and the second leading cause of death in the world. One of the supporting therapeutic efforts to overcome cancer is through food, mainly fruits. Ananas comosus has been investigated for its potential as an anti-cancer. It is a source of antioxidants from the content of Vitamin C and flavonoids that work by capturing free radicals, resulting in inhibition of cancer cell proliferation.Objective :This study was conducted to determine the antioxidant activity and cytotoxicity of water extract of Ananas comosus on T-47D breast cancer cell lines.Method: The process of extracting pineapple flesh is done using water as a solvent, then tested its antioxidant activity using DPPH and cytotoxicity using MTT assay on T-47D.Result: IC50 value for antioxidant activity was 463.369 μg / mL and the IC50 for the cytotoxicity assay was 488.003 μg / mL.Conclusion : The water extract of Ananas comosus have very weak antioxidant activity and moderate cytotoxicity properties that have potential as chemo preventive agents in T-47D breast cancer cell lines.
<p><em>Abstrak - </em><strong>Daging kelinci merupakan salah satu jenis daging yang memiliki potensi tinggi dalam pemenuhan konsumsi daging bagi masyarakat. Daging kelinci memiliki beberapa keistimewaan yaitu kandungan asam lemak jenuh dan kolesterol yang rendah serta kandungan asam lemak tak jenuh yang tinggi. Salah satu produk olahan daging kelinci yang bisa diperkenalkan kepada masyarakat adalah <em>nugget </em>daging kelinci yang diharapkan dapat memiliki manfaat kesehatan untuk menurunkan risiko Penyakit Jantung Koroner. Tujuan dari penelitian ini adalah untuk mengetahui profil asam lemak jenuh dan tak jenuh serta kandungan kolesterol pada <em>nugget </em>daging kelinci <em>New Zealand White (Oryctolagus cuniculus). </em>Desain penelitian yang digunakan adalah deskriptif eksploratif dengan 3 kali pengulangan menggunakan resep yang sama. Profil asam lemak dan kandungan kolesterol diuji menggunakan metode kromatografi gas. Hasil penelitian menunjukkan bahwa <em>nugget </em>daging kelinci mengandung 17 profil asam lemak jenuh dengan kandungan tertinggi yaitu asam palmitat, disusul oleh asam behenik, asam lignoserik, asam miristat, dan asam margarik. Kemudian, untuk profil asam lemak tak jenuh pada <em>nugget </em>daging kelinci mengandung 9 profil asam lemak tak jenuh tunggal dengan kandungan tertinggi yaitu asam oleat, disusul oleh asam nervonat, asam erukat, asam <em>trans </em>9 elaidat, dan asam palmitoleat. Untuk asam lemak tak jenuh ganda terdiri dari 11 profil asam lemak dengan kandungan yang tertinggi yaitu asam linoleat, disusul oleh DHA, dan EPA. Selain asam lemak, <em>nugget </em>daging kelinci juga mengandung kolesterol sebesar 15,12 mg/100 g. Dengan kandungan gizi yang baik, <em>nugget </em>daging kelinci diharapkan dapat menjadi alternatif makanan untuk menurunkan risiko Penyakit Jantung Koroner.</strong></p><p><em>Abstract - </em><strong>Rabbit meat is one type of meat that has high potential in fulfilling meat consumption for the community. Rabbit meat has several advantages is low saturated fatty acid content and cholesterol and high unsaturated fatty acids. One of the processed products of rabbit meat that can be introduced to the community is rabbit meat nuggets that expected has health benefits it can reduce the risk of coronary heart disease. The purpose of this study was to know profile of saturated fatty acids, unsaturated fatty acids, and cholesterol content on </strong><strong>rabbitmeat nuggetsofNewZealandWhite (<em>Oryctolagus cuniculus</em>). </strong><strong>The research design used was descriptive exploratory with 3 repetitions using the same recipe. Profiles of fatty acids and cholesterol content were tested using gas chromatography method. The results showed that rabbit meat nuggets contained 17 profiles of saturated fatty acids with the highest content of palmitic acid, followed by behenic acid, lignoseric acid, myristic acid, and margaric acid. Then, for unsaturated fatty acid profiles on rabbit meat nuggets containing 9 profiles of monounsaturated fatty acids with the highest content, oleic acid, followed by nervonic acid, erucic acid, elaidat trans acid 9, and palmitoleic acid. Polyunsaturated fatty acids consist of 11 fatty acid profiles with the highest content, linoleic acid, followed by DHA, and EPA. Besides fatty acids, rabbit meat nuggets also contain cholesterol of 15,12 mg / 100 g. With a good nutrition content, rabbit meat nuggets are expected to be an alternative food to reduce the risk of coronary heart disease.</strong></p><p><strong><em>Keywords – </em></strong><em>Cholesterol, Profile of saturated and unsaturated fatty acids, Rabbit meat nuggets </em></p>
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