The total cucurbitacin content produced in the different parts of T. cucumerina L. var. cucumerina viz., fruit, stem and leaves with time and temperature was studied during the year 2007-08. The highest amount of cucurbitacins was produced in the month of February, i.e., 0.8, 1.7 and 3.7 w/w % and lowest was in the month of July 1.9, 0.5 and 0.17 w/w % in fruit, stem and leaves respectively. Present study reviles that, Production of cucurbitacin is temperature dependent as the temperature increases cucurbitacins production increased; decrease in the temperature production of cucurbitacins was found decrease. Due to high content of cucurbitacins, this plant may prove itself as a potent hepatoprotective, anti-inflammatory, cytotoxic agent, antifeedant and antimicrobial properties of the plants.
The object ives of this study were to investigate the effect of growth regulators in callus induction, increase of Among all concentrations and combinations, the best results revealed that leaf-derived callus cultured on 2,4-D (3.0mg -l ) + kin (1.0 mg -l ) produced the highest total cucurbitacins content with an optimu m y ield 4.9% w/w and cucurbitacin-E 2.75% w/w at third week.
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