The rice weevil is considered one of the important insects on stored grains. The aim of the current study was to find death and repellant effect of both lemon grass(Cympobogon citrates) and bacterial preparations(Bacillus theuringensis) on Sitophillus oryzae , Where the results of the study indicated , Where the highest rate of killing of larvae was after two weeks reach 100% for the treatment bacteria at two concentrations ,while the treatment by lemongrass was 33.33% on concentration 10% . The highest rate of expulsion for adults was 100% after 48 hours of treatment of lemongrass and the bacterial preparation with concentrations 10 and 5 % Respectively, as for the treatment of expulsion of the insect larvae the study indicated that the highest percentage of expulsion was 100% after two hours of treatment with the powder and bacteria, for both concentrations of the two treatments. Keywords: Bacillus thurengensis , Cympobogon citrates , Sitophillus oryzae
The genus of mosquitoes is also one of the important factors affecting the diversity of bacteria; the aim of the current research is to isolate the types of bacteria from adult mosquitoes that grow near stagnant water ponds in Baghdad Governorate, in order to collect information about bacterial species common in mosquitoes present in the area. MethodsA number of Culicidae mosquito specimens were collected from Abu Ghraib, Baghdad, and the isolation of microorganisms from mosquitoes after prepared culture media and then a biochemical study of the identification characteristics of bacterial isolates was done. ResultSome morphological traits were studied, and their production of catalase and oxidase enzymes was determined; 2×10 3 bacterial cells/ml of different genera and species were isolated on a solid agar nutrient medium for insect models. Bacterial colonies appeared with shapes ranging from pinworms to colonies with flat edges, white color, and smooth surfaces, and some types of fungi were isolated with a number of 1×10 2 cells/ml. Bacterial cells were isolated on the optional MacConkey medium, only less than 100 cells/ml when using the direct method, and the number reached 1×10 2 bacterial cells/ml for some models. Many Bacillus isolates were isolated, and the cells that formed the spores appeared in rods of different sizes, and the shape and location of the spores differed from the mother cells, where the isolate Bacillus species appeared in small-sized cells with a medial cylindrical spore and in the form of thin rod-shaped cells with a cylindrical spore. The spores are large, medium in size, and from the mother cell, while the sporophyte took a subterminal position in isolate Bacillus spp. The sporophyte appeared in a barrel shape, the average of the parent cell in the isolate, and the spheroid was mediated by the parent cell, and the cells of the isolate were characterized by their small size and the location of the sporophyte under the two ends where the most isolated were Bacillus, Enterobacteriaceae species. ConclusionSeveral types of bacteria accompanying mosquitoes were isolated, Bacillus spp., Staphylococcus, and Streptococcus faecalis, which cause many diseases in humans and need molecular diagnosis to confirm the isolated species more accurately.
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