We present a low-cost and simple method to fabricate a novel lock-and-key mixer microfluidics using an economic stereolithography (SLA) three-dimensional (3D) printer, which costs less than USD 400 for the investment. The proposed study is promising for a high throughput fabrication module, typically limited by conventional microfluidics fabrications, such as photolithography and polymer-casting methods. We demonstrate the novel modular lock-and-key mixer for the connector and its chamber modules with optimized parameters, such as exposure condition and printing orientation. In addition, the optimization of post-processing was performed to investigate the reliability of the fabricated hollow structures, which are fundamental to creating a fluidic channel or chamber. We found out that by using an inexpensive 3D printer, the fabricated resolution can be pushed down to 850 µm and 550 µm size for squared- and circled-shapes, respectively, by the gradual hollow structure, applying vertical printing orientation. These strategies opened up the possibility of developing straightforward microfluidics platforms that could replace conventional microfluidics mold fabrication methods, such as photolithography and milling, which are costly and time consuming. Considerably cheap commercial resin and its tiny volume employed for a single printing procedure significantly cut down the estimated fabrication cost to less than 50 cents USD/module. The simulation study unravels the prominent properties of the fabricated devices for biological fluid mixers, such as PBS, urine and plasma blood. This study is eminently prospective toward microfluidics application in clinical biosensing, where disposable, low-cost, high-throughput, and reproducible chips are highly required.
In this study, we propose an automatic classification of three common types of lymphoma: (1) lymphoma, (2) benign lesion, and (3) carcinoma using lymphoma cell images magnified by 100x and by 400x. A comparative study was performed to find the best architecture to classify lymphoma cell images using the Keras library in Tensorflow. The architectures used in this study are ResNet50, MobileNetV1, and VGG16. Based on the accuracy of lymphoma classification for each architecture, the MobileNet model had the highest accuracy in all three classes at both 100x and 400x magnification levels, which suggests that MobileNet is the best model for lymphoma cell classification. This study can be later used as the base argument in modifying the MobileNet architecture further to get more accurate results in future similar studies.
We present a low-cost and simple method to fabricate microfluidic modules directly on a submillimeter scale utilizing an economic stereolithography (SLA) three-dimensional (3D) printer, which only costs less than 400 USD for the investment. The proposed study is promising for a high throughput fabrication module, typically limited by conventional microfluidic fabrications, such as photolithography and polymer casting methods. We demonstrate the two lock-and-key modular fluidic platforms for connector modules and chamber modules with optimized parameters, such as exposure condition and printing orientation. In addition, optimization of post-processing was performed to investigate the reliability of the fabricated hollow structures, which are fundamental to creating a fluidic channel or chamber. We found out that by using an inexpensive 3D printer, the fabricated resolution can be pushed down to 850 µm and 550 µm size for squared- and circled-shaped, respectively; by the gradual hollow structure, applying vertical printing orientation. These strategies opened up the possibility of developing straightforward microfluidic platforms that could replace conventional microfluidics mold fabrication methods, such as photolithography and milling, which are costly and time-consuming. Considerably cheap commercial resin and its tiny volume employed for a single printing procedure extremely cut down the estimated fabrication cost to less than 50 cents USD/module. The simulation study unravels the prominent properties of the fabricated devices for biological fluid mixers such as plasma blood. This study is eminently prospective toward microfluidics application in clinical biosensing, where disposable, low-cost, high-throughput, and reproducible chips are highly required.
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