Objective: Depending on the antioxidant as well as anti-inflammatory properties of Matricaria Chamomile (MC), the current study aimed to investigate if MC could protect testicles against methotrexate (MTX)-induced testicular damage. Methods: 24 male Wistar albino rats were randomly separated into four groups (n=6/each) as follows. G1, the control group was given a single oral daily dose of distilled water (D.W) for 30 days; G2, (MTX group) rats were administered a single oral daily dose of D.W. and in day 28 the animals have received a single IP dosage of MTX; G3 six rats were given an aqueous extract of chamomile flowers at a dose of 200 mg/kg dissolved in 1 ml D.W. and on day 28 the rats have received a single intraperitoneal dose of MTX, and the last group was six rats received a methanolic extract of chamomile flowers at a dose of 200 mg/kg dissolved in 1 ml D.W. and on the day 28 the animals were received a one dosage intraperitoneal of MTX. At the end of the experiment, all animals were anesthetized and sacrificed, blood was also collected for the biochemical test as well as the testicular tissues were removed for histology examination. Results: According to biochemical assessments, notably there was no significant difference between the MTX and the other studied groups. In terms of histological examinations of animals treated with MTX, several pathological changes including vacuolization of seminiferous tubules with a reduced population of germinal epithelium were seen as compared to the control. Animals of G3 and G4 (pre-treatment with equates and alcoholic extract of MC before MTX administration) also showed some notable histopathological changes including disorganization of seminiferous tubules and tubular vacuolation. Conclusion: When comparing the control group, examination of testes with H&E staining showed that pre-treatment with Matricaria Chamomile did not affect MTX-induced testicular damage.
The accumulation of mutations across the genome, notably in microsatellite (MS) DNA repeat sequences, is a hallmark of MSI/dMMR tumors. Microsatellite instability (MSI) is a genetic change in which microsatellites, which typically have one to six nucleotide repetitions, acquire mutations corresponding to small nucleotide deletions or insertions. Immunohistochemistry or a PCR-based test directed against a set of five MS areas were used to create an MSI detection method. MSI is a significant biomarker for cancer diagnosis, prognosis, and treatment options.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.