This research was carried out to assess the ability of L. monocytogenes for adhesion and growth in bio lm on stainless steel coupons under different stressing conditions (NaCl, curing salts and quaternary ammonium compounds -QAC), besides determining the expression of different genes involved in bio lm formation and stress response. Results from crystal violet assay revealed that one isolate carrying a premature stop codon (PMSC) in agrC gene formed high-density bio lms in the presence of QAC or cure salts (7.5% and 10%). Reverse Transcriptase-qPCR results revealed that isolates of L. monocytogenes lineages I and II presented differences in transcriptional pro le of genes related to bio lm formation and adaptation to environmental conditions. In conclusion, our results demonstrated how L. monocytogenes can survive, multiply and form bio lm under adverse conditions related to food processing environments. Differences in transcriptional expression were observed, highlighting the role of regulatory gene networks for particular serotypes under different stress responses.
Significance and Impact of the Study: Listeria monocytogenes, a well-known foodborne pathogen and the causative agent of listeriosis, is highly persistent in food processing environments due to its high adhesion potential in different surfaces. In this study, we aimed to assess how the main stressing conditions usually observed in meat processing facilities (sanitizers, NaCl, curing salts) interfere in L. monocytogenes adhesion and subsequent biofilm development. The development of biofilms by L. monocytogenes was enhanced in the presence of quaternary ammonium-based sanitizer at 4°C.
This research was carried out to assess the ability of L. monocytogenes for adhesion and growth in biofilm on stainless steel coupons under different stressing conditions (NaCl, curing salts and quaternary ammonium compounds - QAC), besides determining the expression of different genes involved in biofilm formation and stress response. Results from crystal violet assay revealed that one isolate carrying a premature stop codon (PMSC) in agrC gene formed high-density biofilms in the presence of QAC or cure salts (7.5% and 10%). Reverse Transcriptase-qPCR results revealed that isolates of L. monocytogenes lineages I and II presented differences in transcriptional profile of genes related to biofilm formation and adaptation to environmental conditions. In conclusion, our results demonstrated how L. monocytogenes can survive, multiply and form biofilm under adverse conditions related to food processing environments. Differences in transcriptional expression were observed, highlighting the role of regulatory gene networks for particular serotypes under different stress responses.
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