Objetivou-se analisar durante o triênio 2012-2014 as ocorrências de acidentes de trânsito ocasionados por animais nas rodovias federais do estado de Pernambuco, com base em informações obtidas no banco de dados do sistema operacional da Polícia Rodoviária Federal, o Siger2. Foram analisadas as variáveis: quantitativo de acidentes por ano, tipo de veículo, rodovia de ocorrência, tipo de pista, traçado da via, condições meteorológicas, fase do dia, tipo do solo (perímetro da via), sexo, idade dos condutores e estado físico das vítimas. No triênio de 2012 a 2014 foram registrados 1.013 acidentes do tipo atropelamento animal. Constatouse maior ocorrência de acidentes no ano de 2012 (40,1%), ocasionados por automóveis (46,9%), na BR 232 (37,5%), em pista simples (72,8%) com trechos em linha reta (92,8%), em céu claro (67,4%), fase de plena noite (65,7%), e no perímetro rural (72,2%). Predominaram os condutores do sexo masculino (86,3%), com idade entre 18 a 40 anos (54,5%) e 63,3% das vítimas foram classificadas como ilesas. Considera-se fundamental a implementação de políticas públicas e medidas mitigatórias que forneçam subsídios para melhorias na conservação e sinalização das rodovias, bem como a elaboração de programas destinados a minimizarem o acesso dos animais as rodovias e campanhas educativas direcionadas aos tutores de animais e condutores, sendo a participação do médico veterinário essencial na elaboração e implementação dessas medidas.
Summary In the present study, the cryoprotective effects of Lolium perenne antifreeze protein (LpAFP) on the vitrification of bovine embryos were evaluated. In vitro-produced blastocysts were divided into two groups: the control group (CG) without the addition of LpAFP and the treatment group (TG) with the addition of 500 ng/ml of LpAFP in the equilibrium and vitrification solution. Vitrification was carried out by transferring the blastocysts to the equilibrium solution [7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO)] for 2 min and then to the vitrification solution (15% EG, 15% DMSO and 0.5M sucrose). The blastocysts were deposited on a cryotop device and submerged in liquid nitrogen. Warming was carried out in three steps in solutions with different sucrose concentrations (1.0, 0.5, and 0.0 M, respectively). Embryos were evaluated for re-expansion/hatching, the total cell count, and ultrastructural analysis. There was no significant difference in the re-expansion rate 24 h after warming; however, there was variation (P < 0.05) in the hatching rate in the TG and the total number of cells 24 h after warming was higher in the TG (114.87 ± 7.24) when compared with the CG (91.81 ± 4.94). The ultrastructural analysis showed changes in organelles related to the vitrification process but, in the TG, there was less damage to mitochondria and rough endoplasmic reticulum compared with the CG. In conclusion, the addition of 500 ng/ml of LpAFP during the vitrification of in vitro-produced bovine embryos improved the hatching rate and total cell number of blastocysts after warming and mitigated intracellular damage.
The objective of this study was to evaluate the profile of the main serum biochemical variables in buffalo heifers receiving diets with different concentrations of concentrate. Twenty buffalo heifers of the Murrah breed, aged six to eight months, were randomly assigned to four groups, with five replicates each, to be adapted to levels of 20%, 40%, 60%, and 80% of concentrate in the diet, composed of sugarcane (bulk) and concentrated feed based on wheat, corn, and soybean meal. The experimental diets were constituted in the proportion "bulk: concentrate", based on dry matter, of "80:20" (Treatment 1), "60:40" (Treatment 2), "40:60" (Treatment 3), and "20:80" (Treatment 4). Biomarkers were evaluated for energy, protein, mineral, and endocrine profiles, as well as enzymatic activity. A positive linear effect was observed for Final Body Weight. In the energy profile, there was a positive linear effect for total cholesterol and HDL cholesterol and a quadratic effect for plasma glucose. In the protein profile, a quadratic effect was observed for the urea: creatinine ratio and a positive linear effect for total protein and albumin. For the enzymatic activity, only the variable gamma-glutamyltransferase presented a quadratic effect. In the mineral profile, a quadratic effect was observed for calcium levels and a positive linear effect for phosphorus levels and negative for chlorine levels. In the endocrine profile, no statistical differences were observed. Diet with until 80% of concentrate does not negatively influence biochemical parameters that represent the profile of energy, protein, mineral and thyroid hormones for growing buffalo heifers and favors the performance of the animals. How to cite this paper: da Costa, C.A., da
There is particular interest in knowledge of the physiological alterations that occur during the transition period, with the aim of understanding the intensity of metabolic alterations and their impacts on animal health. Due to the paucity of data on Moxotó females, related to the dynamics of the energy profile, protein, and mineral metabolites, the present study aimed to evaluate the profile of some blood biomarkers during the transition period. There was significant variation in metabolic dynamics related to energy, protein, and mineral profiles. There is a need for further study of other parameters, rendering them reference values in the different periods of gestation, particularly in the final third of gestation, when metabolic diseases can occur, putting the lives of the animals at risk. ResumoÉ grande o interesse pelo conhecimento das modificações fisiológicas que ocorrem no período de transição, visando conhecer a intensidade das alterações metabólicas e seus reflexos na saúde animais. Mediante a escassez de dados com fêmeas da raça Moxotó relacionado à dinâmica de metabólitos do perfil energético, proteico e mineral, objetivou-se avaliar o perfil de alguns biomarcadores sanguíneos durante os períodos de transição. Verificou-se significativa variação na dinâmica de metabólicos relacionados aos perfis energético, proteico e mineral. Há necessidade de melhor estudar outros parâmetros, tornando-os como valores referenciais nos diferentes períodos da gestação, particularmente no terço final da gestação, quando enfermidades metabólicas podem ocorrer, colocando em risco a vida dos animais.
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