Background: Basal cell carcinoma (BCC) has been proved to have the highest incidence among eyelid malignancies. Basal cell carcinoma is the most common invasive cancer in humans. It is capable of causing extensive tissue destruction. The aim of the study was to identify the characteristics of adult patients with malignant eyelid BCC in Cicendo Eye Hospital Bandung. Methods: A descriptive retrospective study was conducted to determine the characteristics of adult patients with malignant eyelid BCC in Cicendo Eye Hospital Bandung from March 2013 to October 2015. Age and sex distribution of the lesions, location and histopathological results of the tumors were stratified as variables. The inclusion criteria were patients over age of 20, histopathologically diagnosed with periocular BCC. Results: A total of 37 cases histopathologically diagnosed with periocular BCC were evaluated. Females had a slight predominance. The mean age was 61.7 years. The most common site was on the lower lid. The tumors occurred more on the left side. The most common histological subtype was represented by solid growth pattern.
Lacrimal drainage obstruction is a common disorder of lacrimal system. This obstruction could affect lacrimal punctum, canaliculi, lacrimal sac, and lacrimal duct. The etiology could be congenital or acquired, such as triggered by infection or other conditions. This case series illustrates the procedure of canaliculotomy to reduce the epiphora symptom caused by lacrimal drainage obstruction, specifically in punctum and canaliculi. The aim of this case series was to report the management of punctal and canaliculi obstruction with canaliculotomy procedure. Case 1, an eighty-two years old woman with chronic canaliculitis on the right eye due to Actynomyces sp. infection, underwent canaliculotomy and curetage of canalicular area of the right eye. Case 2, a sixty-years old man with chronic canaliculitis with canalicular fistula on the left eye underwent canaliculotomy with silicone tube procedure on the left eye. Case 3, a sixty-seven years old man with recurrent punctal obstruction due to punctal stenosis of the left eye, previous punctoplasty, underwent canaliculotomy with silicone tube. Identification of the patient's symptoms is very important in determining the diagnosis and management plan for the patient. Canaliculotomy procedure with antimicrobial and steroid medication after procedure leads to a good result in reducing symptoms caused by lacrimal drainage obstruction due to various etiology.
Studies in neuroscience can be performed in vitro and in vivo. In vivo studies will show significant results, but it is difficult to do and time-consuming. Primary hippocampal cell culture widely has used in neurobiological studies such as identifying the cellular mechanism of proteins, neuronal activity, and characteristics. The results of studies conducted on this cell culture will be very useful in discovering pathogenesis of a disease, the effect of a substance on the neuron, and neural basis of memory and learning. However, currently in Indonesia, primary hippocampal cell culture is still rare and difficult to do. The purpose of this study was to demonstrate that primary hippocampal cell culture can be done and developed in Indonesia and to review the application of it in medical researches. The study was an experimental study by obtaining neurons from animal’s hippocampus was conducted in 2015–2018 at Department of Cell Biology, Graduate School of Medicine Osaka University and Faculty of Medicine Universitas Padjadjaran. The experimental animal was mice embryo gathered 17.5-days postcoitus. Enzymatic and mechanical methods collected primary hippocampal cells. The cells counted and cultured, which later were observed to see neuron differentiation. The average number of culture cells from 3 embryonic’s hippocampus were 2.39×106. Neuron differentiation observed on the first day and more visible and numerous on the third day after plating. In conclusion, primary hippocampal cell culture using hippocampus from one hemisphere of embryonic mice brain showed a sufficient number of cells to carry out research and showed neuron differentiation. KULTUR SEL PRIMER HIPOKAMPUS DAN PENGGUNAANNYA DALAM RISET KEDOKTERANPenelitian dalam neurobiologi dapat dilakukan secara in vitro dan in vivo. Penelitian secara in vivo sangat berdampak hasilnya, namun sulit dan memakan waktu yang lama. Kultur sel primer hipokampus banyak digunakan dalam penelitian neurobiologi seperti melihat mekanisme protein seluler, serta aktivitas dan karakteristik neuron. Hasil penelitian yang dilakukan pada kultur sel ini akan sangat bermanfaat dalam menemukan proses suatu penyakit, efek suatu zat terhadap sel saraf, dan kemampuan belajar serta memori. Akan tetapi, saat ini di Indonesia kultur sel primer hipokampus masih jarang dan sulit dilakukan. Tujuan penelitian ini adalah menunjukkan bahwa kultur sel hipokampus primer dapat dilakukan dan dikembangkan di Indonesia, serta meninjau penerapannya dalam riset kedokteran. Penelitian ini merupakan studi eksperimental dengan mengoleksi neuron dari hipokampus hewan coba yang dilakukan pada tahun 2015–2018 di Department of Cell Biology, Graduate School of Medicine Osaka University dan Fakultas Kedokteran Universitas Padjadjaran. Hewan coba berupa embrio mencit hari ke-17,5 pascakoitus. Sel primer hipokampus dikoleksi untuk dihitung dan dikultur menggunakan metode enzimatik dan mekanik. Observasi neuron pada kultur dilanjutkan dengan mengamati diferensiasi neuron. Rerata jumlah sel kultur dari 3 hipokampus adalah 2,39×106. Diferensiasi neuron sudah tampak pada hari pertama dan makin jelas serta tampak pada hari ketiga pascapenanaman. Simpulan, kultur sel primer hipokampus menggunakan hipokampus dari salah satu sisi hemisfer otak menunjukkan jumlah sel yang cukup untuk melakukan suatu penelitian dan menunjukkan diferensiasi dari neuron.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.