Objective. To evaluate pharmacy students' knowledge and confidence regarding penicillin allergy assessment and skin testing (PAAST) before and after a focused didactic instruction and simulation (FDIS). Methods. A multicenter, quasi-experimental, cross-sectional survey study was performed among pharmacy students before and after FDIS on PAAST at two schools of pharmacy. The FDIS on PAAST consisted of an infectious disease faculty-led seminar, student-led penicillin allergy counseling interviews, penicillin skin testing simulation, and case studies to assess penicillin allergy scenarios and management. An anonymous, voluntary, electronic survey was distributed to students (n=159) before and after the FDIS. The pre-and post-intervention survey contained ten PAAST knowledge-based questions and multi-step, 5-point Likert scale statements related to confidence of PAAST. The postintervention survey also evaluated students' perceptions of the FDIS on PAAST. Descriptive statistics were performed, and the Student t-test was used to compare pre-and post-intervention responses. Results. One-hundred and forty-three surveys were completed resulting in a survey response rate of 90%. PAAST knowledge scores (mean±SD) increased overall following the FDIS on PAAST (6.67±1.51 vs. 7.81±1.39). Knowledge scores increased considerably for questions related to penicillin allergy consequences, cross-reactivity, and correct steps of PAAST. Pharmacy students' PAAST confidence scores (mean±SD) also improved following the interactive instruction and simulation (2.30±0.70 vs. 3.22±0.67) with considerable confidence increases in penicillin skin testing. Pharmacy students' perceptions of the FDIS on PAAST were also positive overall. Conclusion. Pharmacy students' knowledge and confidence of PAAST improved following FDIS. This may be an effective strategy to implement PAAST education during pharmacy school.
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