No abstract

Spatial separation of speech and noise in an anechoic space creates a release from masking that often improves speech intelligibility. However, the masking release is severely reduced in reverberant spaces. This study investigated whether the distinct and separate localization of speech and interference provides any perceptual advantage that, due to the precedence effect, is not degraded by reflections. Listeners' identification of nonsense sentences spoken by a female talker was measured in the presence of either speech-spectrum noise or other sentences spoken by a second female talker. Target and interference stimuli were presented in an anechoic chamber from loudspeakers directly in front and 60 degrees to the right in single-source and precedence-effect (lead-lag) conditions. For speech-spectrum noise, the spatial separation advantage for speech recognition (8 dB) was predictable from articulation index computations based on measured release from masking for narrow-band stimuli. The spatial separation advantage was only 1 dB in the lead-lag condition, despite the fact that a large perceptual separation was produced by the precedence effect. For the female talker interference, a much larger advantage occurred, apparently because informational masking was reduced by differences in perceived locations of target and interference.

Treatment of Arabidopsis (Arabidopsis thaliana) alternative oxidase1a (aox1a) mutant plants with moderate light under drought conditions resulted in a phenotypic difference compared with ecotype Columbia (Col-0), as evidenced by a 10-fold increase in the accumulation of anthocyanins in leaves, alterations in photosynthetic efficiency, and increased superoxide radical and reduced root growth at the early stages of seedling growth. Analysis of metabolite profiles revealed significant changes upon treatment in aox1a plants typical of combined stress treatments, and these were less pronounced or absent in Col-0 plants. These changes were accompanied by alteration in the abundance of a variety of transcripts during the stress treatment, providing a molecular fingerprint for the stress-induced phenotype of aox1a plants. Transcripts encoding proteins involved in the synthesis of anthocyanins, transcription factors, chloroplastic and mitochondrial components, cell wall synthesis, and sucrose and starch metabolism changed, indicating that effects were not confined to mitochondria, where the AOX1a protein is located. Microarray and quantitative reverse transcription-polymerase chain reaction analysis revealed that transcripts typically induced upon stress treatment or involved in antioxidant defense systems, especially chloroplast-located antioxidant defense components, had altered basal levels in untreated aox1a plants, suggesting a significant change in the basal equilibrium of signaling pathways that regulate these components. Taken together, these results indicate that aox1a plants have a greatly altered stress response even when mitochondria or the mitochondrial electron transport chain are not the primary target of the stress and that AOX1a plays a broad role in determining the normal redox balance in the cell.

Plant mitochondria contain non-phosphorylating bypasses of the respiratory chain, catalysed by the alternative oxidase (AOX) and alternative NADH dehydrogenases (NDH), as well as uncoupling (UCP) protein. Each of these components either circumvents or short-circuits proton translocation pathways, and each is encoded by a small gene family in Arabidopsis. Whole genome microarray experiments were performed with suspension cell cultures to examine the effects of various 3 h treatments designed to induce abiotic stress. The expression of over 60 genes encoding components of the classical, phosphorylating respiratory chain and tricarboxylic acid cycle remained largely constant when cells were subjected to a broad range of abiotic stresses, but expression of the alternative components responded differentially to the various treatments. In detailed time-course quantitative PCR analysis, specific members of both AOX and NDH gene families displayed coordinated responses to treatments. In particular, the co-expression of AOX1a and NDB2 observed under a number of treatments suggested co-regulation that may be directed by common sequence elements arranged hierarchically in the upstream promoter regions of these genes. A series of treatment sets were identified, representing the response of specific AOX and NDH genes to mitochondrial inhibition, plastid inhibition and abiotic stresses. These treatment sets emphasise the multiplicity of pathways affecting alternative electron transport components in plants.

Children 2, 2 1/2, and 3 years of age engaged in a search task in which they opened 1 of 4 doors in an occluder to retrieve a ball that had been rolled behind the occluder. The correct door was determined by a partially visible wall placed behind the occluder that stopped the motion of the unseen ball. Only the oldest group of children was able to reliably choose the correct door. All children were able to retrieve a toy that had been hidden in the same apparatus if the toy was hidden from the front by opening a door. Analysis of the younger children's errors indicated that they did not search randomly but instead used a variety of strategies. The results are consistent with the Piagetian view that the ability to use representations to guide action develops slowly over the first years of life.

The emergence of Arabidopsis as a model plant provides an opportunity to gain insights into the role of the alternative oxidase that cannot be as readily achieved in other plant species. The analysis of extensive mRNA expression data indicates that all five Aox genes (Aox1a, 1b, 1c, 1d and 2) are expressed, but organ and developmental regulation are evident, suggesting regulatory specialisation of Aox gene members. The stress-induced nature of the alternative pathway in a variety of plants is further supported in Arabidopsis as Aox1a and Aox1d are amongst the most stress responsive genes amongst the hundreds of known genes encoding mitochondrial proteins. Analysis of genes co-expressed with Aoxs from studies of responses to various treatments altering mitochondrial functions and/or from plants with altered Aox levels reveals that: (i) this gene set encodes more functions outside the mitochondrion than functions in mitochondria, (ii) several pathways for induction exist and there is a difference in the magnitude of the induction in each pathway, (iii) the magnitude of induction may depend on the endogenous levels of Aox, and (iv) induction of Aox can be oxidative stress-dependent or -independent depending on the gene member and the tissue analysed. An overall role for Aox in re-programming cellular metabolism in response to the ever changing environment encountered by plants is proposed.

Young children's strategies were evaluated as they grasped and used objects. Spoons containing food and toys mounted on handles were presented to 9-, 14-, and 19-month-old children with the handle alternately oriented to the left and right. The alternating orientations revealed strategies that the children used for grasping items. Younger children usually reached with their preferred hand, disregarding the item's orientation. In the case of the spoon, this strategy produced awkward grasps that had to be corrected later. Older children anticipated the problem, alternated the hand used, and achieved an efficient radial grip (i.e., handle grasped with base of thumb toward food or toy end) for both orientations. A model of the development of action-selection strategies is proposed to illustrate planning in children younger than 2 years.

The alternative oxidase (AOX) is found in all plants examined to date, fungi and lower invertebrates. We propose that AOX is not only part of the stress response in plants, but it also plays a central role in defining the stress response. Three lines of evidence support this proposal: (1) The absence of AOX leads to an alteration of stress defences in normal and stress conditions, (2) the expression of AOX is triggered by a variety of signals indicating that it is a common response and (3) AOX acts as a buffer that determines the threshold for the induction of programmed cell death. Therefore, AOX is not only one of many components involved in the defence response, its activity or lack of activity leads to a radical alteration of the defence equilibrium at a cellular level and thus it plays a central role in programming the stress response. This programming role of AOX can be achieved directly by its ability to suppress the induction of reactive oxygen species (ROS) and indirectly by causing changes in the energy status of cells owing to the non-phosphorylating nature of the alternative respiratory pathway. The latter is likely achieved in combination with a variety of alternative NAD(P)H dehydrogenases, that are co-regulated with AOX. Additionally, we explore the possible function of AOX as a component of the stress response beyond the plant frontier.