Soybean (Glycine max) is a major economic crop in Tennessee. In August 2017, foliar interveinal chlorosis progressing to necrosis and root necrosis symptoms consistent with taproot decline, caused by Xylaria sp. (Allen et al., 2017), were observed in a production field of ‘Croplan 4886’ soybean cultivar (WinField Solutions, LLC) in Hardeman County, Tennessee. Characteristic stromata were observed in the field at the base of infected plants, with some produced on cotton debris from the previous season. Infected roots of 13 plants were collected, surface sterilized in the laboratory by rinsing in tap water for 10 minutes, immersed in 10% NaOCl for 1 minute, rinsed twice in sterile distilled water, split longitudinally, and plated on potato dextrose agar amended with 0.125 g/L streptomycin sulfate salt and 0.075 g/L chloramphenicol (PDA-CS) (Allen et al., 2017). Plates were incubated at 22°C on a 12-hr fluorescent light:dark cycle for 1-2 weeks. Based on colony morphology, two isolates were selected for subsequent analyses (DMCC2477 and DMCC2478). In order to fulfill Koch’s postulates, both isolates were used to inoculate the soybean cultivar Asgrow 4632 (Monsanto Co., St. Louis, MO). Inoculum was grown on sterilized soybean stems for one month prior to inoculation. Three treatment groups (non-treated check, sterilized soybean stems, and inoculated soybean stems) were included with four replicates each, where three plants per pot was a replicate. Six seeds were initially planted in 15-cm pots in potting mix (Pro-Mix BX, Premier Horticulture Inc., Canada) and weeded thinned to 3 plants after emergence. Plants were watered every 1-2 days, supplemented with fertilizer, and grown under supplemental lighting with a 16-hr light:8-hr dark cycle with temperatures ranging from 25-35°C in a greenhouse. Foliar symptoms including wilting and interveinal chlorosis followed by necrosis were observed 3-4 weeks after inoculation on the majority of inoculated plants. No symptoms were observed on plants treated with sterilized soybean stems or non-treated plants. This experiment was repeated twice. DNA from original isolates, DMCC2477 and DMCC2478 was amplified with PCR using universal primers targeting four loci, ⍺-actin (ACT), nuclear ribosomal internal transcribed spacer (nrITS), RNA polymerase subunit II (RPB2), and β-tubulin (TUB2). DNA sequences were obtained with Sanger sequencing and deposited in GenBank (nrITS: MH046898, MH046900; ACT: MH113624, MH113625; RPB2: MH113626, MH113627; TUB2: MH113628, MH113629). Roots from symptomatic inoculated plants that had mycelial growth in the pith after 3-4 weeks were used to obtain pure cultures as described above and these were sequenced for the nrITS region to confirm presence of the fungus (GenBank accession numbers: MH046899, MH046901). Alignment of sequences of each loci with published sequences of taxa in the family Xylariaceae (Hsieh et al., 2010; U’ren et al., 2016) revealed both isolates DMCC2477 and DMCC2478 (and re-isolations) were in the Xylaria arbuscula aggregate and were conspecific with the first isolate identified as the causal pathogen of taproot decline, MSU_SB201401 (Allen et al., 2017) (Figure 1). To our knowledge, this is the first report of taproot decline in Tennessee, an emerging disease previously reported in Alabama, Arkansas, Louisiana, Mississippi, and Missouri (Allen et al., 2017). This disease has been shown to be economically significant to soybean production, threatening the ~1.64 M acres planted annually in Tennessee (USDA, 2015-2019). Education and monitoring efforts should be provided to Tennessee producers regarding the detection of this disease and management options.
In November of 2013, a specimen of Japanese sleeper ray, Narke japonica (Temminck et Schlegel), caught off Nanfang-ao, Taiwan was found to be parasitised by the cestode Anteropora japonica (Yamaguti, 1934). Specimens comprised whole worms and free proglottids, both of varying degrees of maturity. This material allowed for the opportunity to examine in detail the developmental progression of this hyperapolytic lecanicephalidean species with regard to overall size, scolex dimensions, and microthrix pattern. Complete immature worms ranged in size from 2.4 mm to 14 mm. The smallest scoleces were half as wide as larger scoleces and exhibited a much smaller ratio of apical organ width to bothridial width. Proglottids more than quadrupled in length during maturation from terminal attached immature to detached proglottids. In addition, a change in microthrix pattern was observed on the anterior region of the proglottids from immature to gravid proglottids; the anterior region of attached immature proglottids is covered with gladiate to coniform spinitriches with capilliform filitriches only rarely visible, whereas this region in detached proglottids is covered with gladiate to coniform spinitriches and conspicuous capilliform filitriches. This is the first report of A. japonica from outside Japan expanding its distribution south to Taiwan. In addition, a preliminary phylogenetic analysis of the genus is presented that suggests congeners from the same host species are not each other's closest relatives, nor is there an apparent phylogenetic signal for apical organ type or reproductive strategy (apolysis). However, reproductive strategy does seem to be correlated with host group such that euapolytic species parasitise dasyatid stingrays while hyperapolytic species parasitise either torpediniform rays or orectolobiform sharks.
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