In the version of this article initially published, the molecular-weight cutoff of the filtering unit was incorrectly given as 10,000 kDa; the correct value is 10 kDa. The incorrect value was given in Methods section "Generation of fluorescently labeled nanobodies, " in the sentences beginning "Purity of recombinant nanobody was assessed by SDS-PAGE ... " and "Subsequently, the unbound fraction was added .... " In the same section, the buffer composition for the hepta-mutant sortase incorrectly included 10 mM CaCl 2. The correct text is "To this end, purified GGGC-AF594 (80 μM) was incubated with purified nanobody-LPETGG-6×His (5 μM) and penta-mutant (5 M) or hepta-mutant (7 M) sortase (0.8 μM) for 2 h at 4 °C in 50 mM Tris pH 8 and 150 mM NaCl, and in the case of the penta-mutant, 10 mM CaCl 2 was added (sortase was produced in house according to a previously described protocol using sonification instead of French press 40). The errors have been corrected in the HTML and PDF versions of the article.
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