New Zealand white rabbits were administered soluble lipoteichoic acid from Streptococcuspyogenes 1-RP41 on alternate days for up to 30 days. An increased incidence of renal cortico-medullary calculi was observed after day 21; the use of fluorescent-labeled anti-teichoic acid antibody located teichoic acid predominantly in the cortical-associated tubules.
A factor promoting tumor growth in allogeneic mice was found in the ascites fluid of TA3-Ha tumor-bearing mice. Preliminary attempts t o isolate this factor resulted in a preparation (P3), devoid of free immunoglobulins or serum proteins, which had enhanced action in promoting tumor growth in vivo. Electron microscopy showed that the P3 preparation is of membranous origin. Biochemical assays for membrane marker enzymes were positive. 5'-Nucleotidase and alkaline a-glycerophosphatase activity was found. Chemical and gas-liquid chromatographic analysis of long-chain carboxylic acids present in the tumor cell membrane and in the P 3 revealed the presence of fatiy acids in almost identical ratios in both preparations, again indicating the membranous character of P3.The P3 preparation as well as the ascites fluid have immunogenic properties. They could induce immune tolerance if injected neonatally and the P3 preparation could immunize against the TA3-Ha tumor if injected intramuscularly or subcutaneously into adult mice. It had no effect if injected intravenous- A working hypothesis has been proposed according t o which TA3-Ha tumor cells produce highly reactive membranous decay products which intercept incoming immunocytes o r immunoglobulins before they can reach their targets o n living tumor cells. Whether this self protection of TA3 ascites tumors can be applied to other tumors remains t o be investigated.ly.
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