The dwarf rose 'Victory Parade' meristem culture was initiated and propagated on the Murashige, Skoog (M.S.) basal medium supplemented with benzyladenine (BA;1.0 mg -1---'), indolylbutyric acid (IBA; 0.001 mg-1-"'), and ascorbic acid (5 mg -1-').The elongation medium was 1/2 M.S. supplemented with kinetin (0.05 mg-1-!), indolylacetic acid (lAA; 1.0 mg-1-'), and ascorbic acid (5 mg-1-'). Woody plant medium (WPM) supplemented with TAA (0.2 mg-1-') was the most effective for rooting. Roots could be initiated after the transfer of plantlets from the elongalion or proliferation medium to an unsterile substrate soaking them for 16 h in 1/3 WPM supplemented with IBA (25 mg:l-') or moistening the substrate with 1/3 WPM supplemented with IBA (10 mg-1-1).
A rare bisexual plant of Actinidia kolomikta (variety 'lisaku') was introduced into Estonia from the Far East (from the vicinity of the Botanical Garden of Vladivostok), and it was micropropagated in order to preserve its genetic potential. The culture was initiated from 1 cm long single node stem cuttings on Murashige & Skoog basal medium (MS) + 6-benzyladenine (BA) (1.0 mg/1) + indolebutyric acid (IBA) (0.01 mg/l) and multiplicated on WMS (Woody Plant Medium macroelements + MS microelements and vitamins) + BA (1.0 mg/l) + IBA (0.1 mg/l). On this medium shoot multiplication occurred at a rate of 7 folds after 4 weeks. Shoots were elongated on 1/2 MS + kinetin (0.1 mg/l) + indoleacetic acid (lAA) (1.0 mg/l) and rooted on the medium 1/2 MS + lAA (0.2 mg/l). The shoots could be directly rooted in vivo with up to 89.3% survival.
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