A capillary electrophoresis detection technique for (small) peptides is presented, i.e. quenched phosphorescence, a method that is generally applicable and does not require chemical derivatization. For this purpose, a novel phosphorophore, 1-bromo-4-naphthalenesulfonic acid (BrNS), was synthesized. BrNS has sufficient water solubility and provides strong phosphorescence at room temperature over a wide pH range. The detection is based on the dynamic quenching of the BrNS phosphorescence background signal by electron transfer from the amino group of the peptides at pH 9.5-10. For the di- and tripeptides Val-Tyr-Val, Val-Gly-Gly, Ala-Ser, Gly-Asn, Gly-Ala, and Gly-Tyr, detection limits in the range of 5-20 microg/L were obtained. The novel technique is even a good alternative for the (limited) group of peptides containing tyrosine and, thus, exhibiting native fluorescence as well as strong UV absorption: using Gly-Tyr, Val-Tyr-Val, methionine enkephalin, and human angiotensin II as test compounds, quenched phosphorescence detection was found to compare favorably with absorption detection at 190- and 266-nm laser-induced fluorescence detection, as performed with a recently developed, small-size, quadrupled Nd:YAG laser.

We present the Virtual Data Visualizer, a highly interactive, immersive environment for visualizing and analyzing data. VDV is a set of tools for exploratory data visualization that does not focus on just one type of application. It employs a data organization with data arranged hierarchically in classes that can be modified by the user within the virtual environment. The class structure is the basis for bindings or mappings between data variables and glyph elements, which the user can make, change, or remove. The binding operation also has a set of defaults so that the user can quickly display the data. The VDV requires a user interface that is fairly complicated for a virtual environment. We have taken the approach that a combination of more-or-less traditional menus and more direct means of icon manipulation will do the job. This work shows that a useful interface and set of tools can be built. Controls in VDV include a panel for controlling animation of the data and zooming in and out. Tools include a workbench for changing the glyphs and setting glyph/variable ranges and a boundary tool for defining new classes spatially.

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