High resolution two-dimensional electrophoresis combined with a sensitive silver stain has been used to study the proteins of normal human aqueous humour. The protein patterns of small, unconcentrated, individual samples and of pooled and concentrated aqueous humour samples were compared with plasma or serum from the same individual or with a normal serum. The protein maps of aqueous humour and serum showed great similarity. More than 30 groups of spots were visualized in aqueous humour after silver staining and 18 of them have been tentatively identified. Three polypeptide groups were observed which were not found in plasma or serum. The different clusters, which appeared in the region of high (> 90 000), medium (50 000-45 000) and low (25 000-15 000) molecular weight polypeptides, remain unidentified. The most abundant cluster represents a molecular weight of approximately 48 000.
The Galand lens is a closed J-loop implant for capsular-bag fixation. We present a retrospective study of 214 eyes implanted with this lens. Eyes were examined at three months, six months, one year, and two years after surgery. Results indicate that 82.2% of eyes obtained a visual acuity of 20/40 or better at two years after implantation. If patients with other ocular pathology were excluded, the percentage of those whose visual acuity was 20/40 or better at two years was 93.7%. Although this implant avoids "spring action" within the capsular bag, the rate of dislocation into the posterior chamber was low (0.93% in this series). Early stability, before sealing of the capsular leaflets, was obtained by limiting the anterior capsulectomy to the pupillary area. No late zonular rupture was observed. Secondary posterior capsulotomy has been performed in 9.8% of the eyes.
The use of the operating microscope has become almost a necessity in the surgery of the anterior segment of the eye. However the coaxial light of the operating microscope is potentially hazardous for the retina, especially during intra-ocular lens implantation. The risk of producing a photic retinopathy increases if the duration of the surgical procedure is prolonged once the pseudophakos is in the eye and if high levels of illumination are used. We encountered our first case in a patient, whose intervention was filmed and where thus an increased illumination was needed. This complication is however also found in cases where no extra illumination was needed. As the clinical signs are usually very mild, the retinal lesions related to over-exposure to light are easily overlooked.
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