Highly fluorescing biological labels with excitation in near-infrared window II have attracted the interest of scientific community as they are capable of increasing both penetration depth and imaging quality. However, studies on the utilization of quantum dots (QDs) in biological imaging appear to be rather limited to the near-infrared window I (NIR-I: 650−950 nm). We herein report on the observation of efficient photoluminescence (PL) in Mn 2+ -doped ZnS QDs excited by two-photon absorption (2PA) in near-infrared window II (NIR-II: 1000−1350 nm). Multiphoton-absorptioninduced PL measurements indicate that these biocompatible QDs exhibit a two-photon action cross-section of 265 GM at 1180 nm, the highest value reported to date among conventional fluorescent probes on excitation in NIR-II. This value is 1−2 orders of magnitude higher than that for organic dye molecules excited by NIR-I photons and 3−4 times greater than that of fluorescent proteins excited in the NIR-II. The underlying NIR-II excitation mechanism for the Mn 2+ emission at 586 nm on account of the 4 T 1 − 6 A 1 transition is attributed to the transitions from the valence subband of ZnS QDs (or ground states of Mn 2+ ions) to the excited states of Mn 2+ ions by direct two-photon absorption. Transient PL measurements reveal single exponential decay with a PL lifetime of 0.35 ± 0.03 ms irrespective of excitation wavelength, which are 4−5 orders longer than that of conventional fluorescent probes. With the excitation in NIR-II window and the unique combination of photophysical properties such as a greater two-photon action cross-section, a longer PL lifetime, and larger anti-Stokes shift (450 nm or more), Mn 2+ -doped ZnS QDs appear to be a promising candidate for deep tissue imaging applications.
We
report on the dynamical properties of photoexcited carriers,
particularly the charge transfer, in CdS–CdSe–CdS segmented
nanorods using femtosecond transient pump–probe spectroscopy.
Design of this kind of heteronanostructures with the possibility of
variation of the relative volumes of CdS and CdSe segments permits
independent tuning of one-photon and two-photon absorption cross-sections
over a wide range of wavelengths, with specific advantages in applications
related to photovoltaics and multiphoton microscopy. Intensity-dependent
charge transfer dynamics in CdS–CdSe–CdS segmented nanorods
indicates that the rate of charge transfer from CdS to CdSe is influenced
by the number of electron–hole pairs generated in the nanorod.
We attribute this change in the rate constant to Auger recombination-assisted
charge transfer, which becomes the predominant relaxation mechanism
at high intensities. Charge transfer also results in a large two-photon
absorption cross-section, on the order of 104 GM (1 GM
= 10–50 cm4 s photon–1), at 1.55 eV in these heteronanostructures. Furthermore, two-photon
absorption induced photoluminescence on near-infrared excitation (1.55–0.99
eV) suggests that the local field effects plays a role in determining
the effective two-photon action cross-section of heteronanostructures,
offering a platform for engineering optical nonlinearity.
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