The benzoyl ester of Pluronic L-81 (BEP) has been purified on an aluminium oxide column and fractionated on the basis of its mobility characteristics into three fractions, BEP I, II and III. Absorption studies were made with rats fed by gavage a lipid preparation containing [14C]triolein and [3H]cholesterol (control animals) and one of the fractions or Pluronic L-81, or crude BEP. After 4 h the amount of lipid absorbed and transported was calculated as the difference between the dose fed and the amount of lipid recovered from the gastric and intestinal luminal contents and intestinal mucosa. Pluronic L-81 was the most effective in inhibiting absorption of triolein but it did not inhibit absorption of cholesterol. BEP-II was almost as effective in inhibiting the absorption of triolein and also inhibited absorption of cholesterol. Crude BEP was less effective and BEP-I and III had only limited activity. Inhibition of triolein absorption by Pluronic L-81 may be partly related to its delaying action on gastric emptying. As purified BEP preparations had practically no effect on gastric emptying, their inhibiting activity involved intestinal mechanisms of lipid absorption.
A sensitive sandwich enzyme immunoassay has been developed for quantitation of alpha-amylase in pig pancreas. The alpha-amylase-antibody conjugate was prepared from horseradish peroxidase coupled with antibodies to hog pancreas alpha-amylase. With this method the measuring range is 1-10 ng/ml and the detection limit is 0.2 ng/ml. The amount of pig pancreatic amylase was measured to be 6.21 +/- 2.05 mg/g of tissue. The enzymatic activity determination and immunoassay were compared in the material studied.
Monosaccharide composition was determined in apolipoprotein B-48 (apoB) of chylomicrons of rat mesenteric lymph. Chylomicrons were separated into three fractions based on density. Triglyceride and apolipoprotein content were determined in each. ApoB was isolated and quantified using precipitation with isopropanol. Chylomicrons were collected in lymph under normal conditions, and with Poloxalene 2930 when chylomicron secretion was inhibited. Most of the triglyceride was carried in the least dense fraction, while the highest apoB content was in the most dense fraction under normal conditions. Mannose and galactosamine contents of apoB were similar in all fractions while contents of both glucosamine and galactose were highest in the least dense fraction. When chylomicron secretion was inhibited by Poloxalene, the amount of triglyceride recovered in the least dense fraction was significantly reduced. Despite the inhibition of lipid transport in the least dense fraction of chylomicrons by Poloxalene, there was little change in apoB recoveries and in the relative content of various monosaccharides in the apoB from each of the three fractions as compared to results obtained during lipid absorption under normal conditions. In conclusion, carbohydrate composition of apoB of chylomicrons is heterogeneous and varies with chylomicron density.
After the administration of D-galactosamine to golden Syrian hamsters, a rapid
but short-lived increase in acylase I and cobalt-activated acylase (AA-Co) activity was noted
in the blood plasma, but not in the liver. In the plasma of control hamsters, only form 2
AA-Co was identified, but during experimental hepatitis, the appearence of form 1 was
observed. Only form 2 hamster enzyme is strongly inhibited by α-hydroxyisocaproil-tyrosine
and reacts with antihuman form 2 antibody.
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