From dry-off until 7 d before expected parturition date, 30 Holstein cows were fed a low energy diet (control) to maintain body condition or high energy diets (high fat or isocaloric high grain) to attempt to increase body condition. After parturition, all cows were fed a common lactation diet. Body condition was unchanged for cows fed control or high grain diets, but was decreased for cows fed the high fat diet because DMI was decreased. Cows fed the high fat diet during the dry period had decreased triglyceride accumulation in the liver at 1 d postpartum, which was accompanied by lowered plasma NEFA, increased peroxisomal beta-oxidation of palmitate by liver homogenates, and decreased esterification of palmitate by liver slices. During the last 3 wk prepartum, cows fed the high fat diet had lower glycogen and higher acid-soluble carnitine in liver, higher concentrations of NEFA and bST in plasma, higher ratio of triiodothyronine to thyroxine in plasma, and lower concentrations of insulin and thyroxine in plasma than did cows fed the high grain diet. After parturition, cows fed the high fat diet during the dry period had decreased percentage and production of milk fat; differences among treatments were not significant for DMI, body condition, milk production, or milk protein. Periparturient lipid metabolism was altered significantly in cows fed a high fat diet during the dry period, although diet composition and nutrient intakes were confounded.

Sixteen multiparous Holstein cows averaging 74 d in milk were used in a replicated 4 x 4 Latin square to compare the effects on animal performance of feeding whole plant silage and grain from a glyphosate-tolerant corn hybrid (event NK603), a nontransgenic control hybrid, and two commercial nontransgenic hybrids (DK647 and RX740). The grain and silage from the four corn hybrids were produced using the same procedures and under similar agronomic conditions at the University of Illinois. On a dry matter (DM) basis, diets contained 30% corn silage and 27.34% corn grain produced either from event NK603, a nontransgenic control, or commercial hybrids. Apart from the DM content of silages, the chemical composition of both grain and silage produced from the four corn hybrids were substantially equivalent. Feeding diets that contained event NK603 and DK647 hybrids tended to decrease DM intake (DMI) compared with the control nontransgenic and RX740. The intakes of crude protein (CP), acid and neutral detergent fiber, and nonfiber carbohydrates were not different for cows fed event NK603 and control diets. The RX740 diet resulted in the highest intakes of fiber and CP, whereas the DK647 diet resulted in the lowest intake of CP. These differences in nutrient intake arose from small variations in both the DMI and the chemical composition of feed ingredients and experimental diets. Production of milk and 3.5% fat-corrected milk; milk fat, CP, and true protein percentage and yield; milk urea N; milk total solids percentage and yield; and somatic cell count were not affected by treatments. These data indicate that the stable insertion of the gene that confers tolerance to glyphosate (event NK603) in the corn line used in this experiment does not affect its chemical composition and nutritional value for lactating dairy cows when compared with conventional corn.

Four primiparous Holstein cows in late lactation were used in a 4 x 5 Youden square design. Each cow was fitted with ruminal, duodenal, and ileal cannulas. Treatments were a control diet with a high concentration of forage, a control diet with a low concentration of forage, and three diets in which dried brewers grains replaced a portion of the forage, concentrate, or both. Cows were fed and milked twice daily in 14-d periods. Chromic oxide and Yb (soaked in acid) were used as markers for digesta flow and rate of passage, respectively. No significant differences in ruminal pH or rates of passage caused by treatment were detected. Although brewers grains had slower digestion rates of neutral detergent fiber (NDF) in situ and faster passage rates than did alfalfa, brewers grains had minor effects on site of NDF digestion. Intake of dry matter was depressed by the higher NDF concentrations in diets in which brewers grains replaced concentrate, but a long-term feeding study is needed to verify this conclusion. Although brewers grains did not appear to be very effective at stimulating milk fat percentage, dry matter intake was maintained when brewers grains replaced forage, and it appears that brewers grains can successfully replace a portion of the forage NDF in the diets of lactating cows.