Benzene biodegradation was studied in batch and continuous packed bed bioreactors using polyurethane foam (PUF) as packing media byisolated Bacillus sp. M1. The values of optimized process parameters were found to be 800 × 106 CFU·mL−1, 400 mg·L−1, 7.0 and 37 °C forinoculum size, substrate concentration, pH and temperature respectively. Continuous packed bed bioreactor (CPBBR) was operated and monitoredfor 69 days on laboratory scale at various flow rates (10–60 mL·h−1). The steady state removal efficiency was observed more than 90% up to theinlet load of 288 mg·L−1.d−1 and elimination capacity was found to be 91.2–266.4 mg·L−1·day−1. Monod growth model was applied for the removalof benzene and values were found to be (Ks: 215.07 mg·L−1; μmax: 0.314 day−1).
Efficacy of three different bacterial species for biodegradation of malathion and its secondary products have been investigated. The concentration range of malathion under investigation was 25–200 mg·L−1. It has been observed that Pseudomonas putida was found to be most efficient for degradation of malathion. The removal of malathion was 72% at its concentration of 125 mg·L−1. The optimum parameters were studied for allthree bacterial species in batch mode. The average values of Ks and μmax were obtained for all these species for degradation of malathion. Results indicate that P. putida has high degradation potential than Rhodoccocus rhodochrous and Sphingomonas sp. The degradation of P. putida was maximum at concentration of 125 mg·L−1, pH and temperature at 7 ± 0.2, 80 °C respectively. Metabolites were obtained using GCMS analysis.
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