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By the use of chemically induced dynamic electron polarisation acylphosphine oxides have been shown to undergo the Type I fission reaction from their triplet states.

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Abstract-The limitations and advantages of particular dyes for labelling proteins and other biological materials are discussed. Methods available for conjugating dyes to proteins are outlined. Following a discussion of double labelling methods the use of photoactivatable Ruorochromes and time resolved fluorescence methodologies are outlined. The reasons for the photoinstability of some fluorochromes are discussed and methods for overcoming the problem are described.