Background:To collect the data all allergic responses for different beauty products in different age, sex and people belonging to different socio-economic status. Methods: A survey was done in the local region of Gwalior (India) on the people of different age, gender, and class in terms of allergic response to different beauty products. The areas covered in the survey were beauty parlors, hospitals, and residential colonies. A total of 17 cosmetic products, including soap, lipstick, shaving cream, face cream, and so on, of different brands and usage were selected for study. The survey was conducted through interaction with people, by interviewing and by proving them questionnaires. Result: In result, the ratio of allergic response of the lower class people was comparably high. The maximum percentage of incidence was recorded in people of age group of 36-45 years, whereas minimum percentage in the age group of 56-65 years was recorded. The incidence of allergy in male and female was 60% and 40%, respectively. Conclusion: Allergic response to the beauty product is dependent on above studied factors. Most of the allergic symptoms were noticed in middle age group of lower class society. So, frequent use of cosmetics product should be avoided specially people which have allergic symptom in history.
Bioanalytical method development is the process of creating a procedure to enable a compound of interest to be identified and quantified in a biological matrix. A compound can often be measured by several methods and the choice of analytical method involves many considerations. Analysis of drugs and their metabolites in a biological matrix is carried out using different extraction techniques like liquid-liquid extraction, solid phase extraction (SPE) and protein precipitation from these extraction methods samples are spiked with calibration (reference) standards and using quality control (QC) samples. These methods and choice of analytical method describes the process of method development and includes sampling, sample preparation, separation, detection and evaluation of the results. The developed process is then validated. These Bioanalytical validations play a significant role in evaluation and interpretation of bioavailability, bioequivalence, pharmacokinetic, and toxicokinetic studies. In which different parameters like accuracy, precision, selectivity, sensitivity, reproducibility, and stability are performed. Keywords: - LLE, SPE, Quality control samples, Bioequivalence, Bioavailability, Validation.
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cell that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses. The antibody recognizes a unique molecule of the pathogen, called an antigen, via the fragment antigen binding (Fab) variable region. Each tip of the "Y" of an antibody contains a paratope (analogous to a lock) that is specific for one particular epitope (similarly, analogous to a key) on an antigen, allowing these two structures to bind together with precision. Using this binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize its target directly (for example, by inhibiting a part of a microbe that is essential for its invasion and survival). Depending on the antigen, the binding may impede the biological process causing the disease or may activate macrophages to destroy the foreign substance. The ability of an antibody to communicate with the other components of the immune system is mediated via its Fc region (located at the base of the "Y"), which contains a conserved glycosylation site involved in these interactions. The production of antibodies is the main function of the humoral immune system.
It is internationally recognized that validation is necessary in analytical laboratories. The use of validated methods is important for an analytical laboratory to show its qualification and competency. When analytical method is utilized to generate results about the characteristics of drug related samples it is essential that the results are trustworthy. They may be utilized as the basis for decisions relating to administering the drug to patients. Analytical method validation required during drug development and manufacturing and these analytical methods are fit for their intended purpose. The purpose of this validation is to show that processes involved in the analytical testing can be performed in an effective and reproducible manner. This article provides a good, complete, up-to-date collation of relevant information in the fields of analytical method validation of Trypsin-Chymotrypsin Tablets 50000 AU of Enzymatic Activity. Keywords: Analytical method validation, Pharmaceutical analysis, Specificity, Precision, Accuracy.
Bioanalytical method Validation employed for quantitative determination of drug and their metabolites in biological fluids. Comprises all criteria determining data quality, such as selectivity, accuracy, precision, recovery and senstivity. The main purpose of method validation is to demonstrate that a specific Bioanalytical method can reliably determine the concentration of drug in study sample with high degre of confidence. Validation does not means that method is perfect, but validation means method has met a set of criteria to ensure that it is reliable and consistent. Tizanidine is a central alpha 2 adrenergic agonist –inhibits release of excitatory amino acid in the spinal interneurones. It may facilitate the inhibitory transmitter glycine as well. It inhibits polysyneptic reflexes reduce muscle tone and frequency of muscle spasms without reducing muscle strength. Following oral administration, tizanidine is essentially completely absorbed .The absolute oral bioavailability of tizanidine is approximately 40%, due to extensive first-pass hepatic metabolism. Tizanidine is extensively distributed throughout the body with a mean steady state volume of distribution of 2.4 L/kg following intravenous administration in healthy adult volunteers .tizanidine is approximately 30% bound to plasma proteins. Keywords: Bioanalytical method Validation, LC-MS/MS, Human Plasma, Tizanidine, HPLC.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.