We demonstrated that a behavioural intervention is feasible to deliver and improved obesity measures in AI youth. Future work should evaluate TTP for effectiveness, sustainability and long-term impact in expanded tribal settings.

1. A review of the literature relating to the frequency at which pigs suckle under natural conditions was made. The evidence indicated that the average interval between successive sucklings is approximately 1–1¼ hr.2. A number of sows and their litters were continuously observed for varying periods of time under natural conditions and a similar average interval between sucklings was observed.3. A review of the literature relating to the determination of milk production of sows was made. This showed that the majority of previous workers imposed, during the periods when the litters were being weighed before and after every suckling, a suckling frequency that did not simulate the natural behaviour of the animals. Evidence suggesting that the imposition of such unnatural conditions invalidated the findings, in so far as they might indicate the true milking capacity of the animals, was discussed.4. An experiment to obtain direct evidence on the importance of the suckling interval in relation to the milk obtained by the pigs was carried out. Conclusive evidence was obtained that when suckling was allowed every hour, both the quantity of milk obtained by the litters and their live-weight gain in 24 hr. periods were much greater than was the case when suckling was allowed only every 2½ or 3 hr. In addition, the pigs on the hourly suckling frequency utilized their milk intake more efficiently.5. An experiment was carried out in which an attempt was made to obtain, under natural conditions, a valid estimate of the 56 days' lactation yield of three Large White gilts. The method of weighing the individual pigs before and after suckling was used, and the animals were allowed to suckle every hour.6. The estimated average lactation yield was 768 lb., with a range of from 882 to 655 lb., although it was suggested that the true yield might be some 5–10% higher. The figures obtained in this work were shown to be very much higher than the yields reported by most of the previous workers.7. The nursing and suckling behaviour of the dams and their litters was studied. Observations of significance in relation to the variation in growth of pigs within a litter were made.8. Data were obtained concerning the average amount of milk obtained by individual pigs at a suckling, the total amount ejected at a suckling, the effect of daylight and of darkness on the amount of milk obtained, the relative productivity of the individual mammary glands and the efficiency with which the milk intake was utilized by the individual pigs in the litter.9. A close positive relation between milk intake and live-weight gain during the first 3 weeks of life was found. During the last 5 weeks of lactation, when supplementary food was available, this close relationship was not seen.10. The growth rate of the suckling pig, as related to the supply of food, was discussed. The evidence available suggested that the supply of sow's milk was frequently insufficient to meet the requirements of the pigs for optimum growth.11. The chemical composition of sow's milk in relation to the stage of lactation was studied.

Weight-loss interventions generally improve lipid profiles and reduce cardiovascular disease risk, but effects are variable and may depend on genetic factors. We performed a genetic association analysis of data from 2,993 participants in the Diabetes Prevention Program to test the hypotheses that a genetic risk score (GRS) based on deleterious alleles at 32 lipid-associated single-nucleotide polymorphisms modifies the effects of lifestyle and/or metformin interventions on lipid levels and nuclear magnetic resonance (NMR) lipoprotein subfraction size and number. Twenty-three loci previously associated with fasting LDL-C, HDL-C, or triglycerides replicated (P = 0.04–1×10−17). Except for total HDL particles (r = −0.03, P = 0.26), all components of the lipid profile correlated with the GRS (partial |r| = 0.07–0.17, P = 5×10−5–1×10−19). The GRS was associated with higher baseline-adjusted 1-year LDL cholesterol levels (β = +0.87, SEE±0.22 mg/dl/allele, P = 8×10−5, P interaction = 0.02) in the lifestyle intervention group, but not in the placebo (β = +0.20, SEE±0.22 mg/dl/allele, P = 0.35) or metformin (β = −0.03, SEE±0.22 mg/dl/allele, P = 0.90; P interaction = 0.64) groups. Similarly, a higher GRS predicted a greater number of baseline-adjusted small LDL particles at 1 year in the lifestyle intervention arm (β = +0.30, SEE±0.012 ln nmol/L/allele, P = 0.01, P interaction = 0.01) but not in the placebo (β = −0.002, SEE±0.008 ln nmol/L/allele, P = 0.74) or metformin (β = +0.013, SEE±0.008 nmol/L/allele, P = 0.12; P interaction = 0.24) groups. Our findings suggest that a high genetic burden confers an adverse lipid profile and predicts attenuated response in LDL-C levels and small LDL particle number to dietary and physical activity interventions aimed at weight loss.

Across the Diabetes Prevention Program (DPP) follow-up, cumulative diabetes incidence remained lower in the lifestyle compared with the placebo and metformin randomized groups and could not be explained by weight. Collection of self-reported physical activity (PA) (yearly) with cross-sectional objective PA (in follow-up) allowed for examination of PA and its long-term impact on diabetes prevention. RESEARCH DESIGN AND METHODS Yearly self-reported PA and diabetes assessment and oral glucose tolerance test results (fasting glucose semiannually) were collected for 3,232 participants with one accelerometry assessment 11-13 years after randomization (n 5 1,793). Mixed models determined PA differences across treatment groups. The association between PA and diabetes incidence was examined using Cox proportional hazards models. RESULTS There was a 6% decrease (Cox proportional hazard ratio 0.94 [95% CI 0.92, 0.96]; P < 0.001) in diabetes incidence per 6 MET-h/week increase in time-dependent PA for the entire cohort over an average of 12 years (controlled for age, sex, baseline PA, and weight). The effect of PA was greater (12% decrease) among participants less active at baseline (<7.5 MET-h/week) (n 5 1,338) (0.88 [0.83, 0.93]; P < 0.0001), with stronger findings for lifestyle participants. Lifestyle had higher cumulative PA compared with metformin or placebo (P < 0.0001) and higher accelerometry total minutes per day measured during follow-up (P 5 0.001 and 0.047). All associations remained significant with the addition of weight in the models. CONCLUSIONS PA was inversely related to incident diabetes in the entire cohort across the study, with cross-sectional accelerometry results supporting these findings. This highlights the importance of PA within lifestyle intervention efforts designed to prevent diabetes and urges health care providers to consider both PA and weight when counseling high-risk patients.

SUMMARY1. Twelve blocks of six enzootic-pneumonia-free Large White litter-mate pigs were individually fed, wet, from 20 to 92 kg live weight on six different levels of feed intake. Four groups were fed according to scales based on live weight and two were fed on a ‘semi-ad libitum’ system. One of the scales used was based on the ARC (1967) recommendations.2. Pigs on ‘semi-ad libitum’ feeding grew significantly faster than those on scale feeding although the feed: gain ratios were similar. Differences in performance between the four scale-fed groups were relatively small.3. Although treatment differences in carcass measurements were in the main small, the commercial grading results favoured the carcasses from the scale-fed pigs. The firmness of backfat assessed by thumb pressure was reduced as the level of feeding was increased.4. The results were compared with those obtained in a similar trial carried out at Shinfield in 1957 using pigs of a completely different genetic background. The general conclusions reached were similar in the two trials, that to obtain the most satisfactory overall results some form of controlled scale-feeding was necessary.