We studied the effect of supplementation with vitamins C, E and beta-carotene (PARABION, produced by Syndipharma) on antioxidative status in kidneys of male Wistar rats with diabetes induced by intravenous application of streptozotocin (45 mg.kg-1 of body weight). The animals received subtherapeutic doses of Insulin Interdep (6 U.kg-1 of body weight). A significant decrease of malondialdehyde (MDA), reduced (GSH) and oxidized (GSSG) glutathione and reduction of the activities of Se-glutathione peroxidase (Se-GSH-PX, EC. 1.11.1.9.) and glutathione S-transferase (GST, EC. 2.5.1.18.) were observed in kidneys of diabetic rats treated with these vitamins. On the contrary, the activity of CuZn-superoxide dismutase (CuZn-SOD, EC. 1.15.1.1) and the level of vitamin C (vit. C) increased significantly. No changes were observed for vitamin E (vit. E), beta-carotene and catalase (CAT, EC. 1.11.1.6). Supplementation with vitamins C, E and beta-carotene resulted in an improvement of antioxidative status of kidneys of rats with streptozotocin-induced diabetes.
In mice the oral LD50 values of aluminium chloride and aluminium sulphate were 0-77 ± o0I2 and o098 ± o0og g. Al/kg. (± S.E.) respectively.Mice and rats were fed on a normal diet containing about 170 p.p.m. of aluminium. Doubling this concentration caused a decrease in growth in the second and third generations of mice. A high intake of aluminium sulphate (2,835 p.p.m.) caused a 20-fold increase of aluminium retention in white rats. Aluminium accumulated in various tissues, especially in the skeleton, liver, and testes. A high rate of intake (2,665 p.p.m.) caused a negative phosphorus balance in the rat, with an increased output of phosphorus in the faeces. The lower absorption of phosphorus was also demonstrated with Na2H32PO4. Chronic and acute poisoning by aluminium chloride caused, after intraperitoneal Na2FP2PO4, decreased incorporation of 32p into the phospholipids and nucleic acids of various tissues in the rat. It also caused a fall in the adenosinetriphosphate acid-levels in plasma, and a rise in the adenosine diphosphate level.The results suggest that, the toxic effects of aluminium salts result both from decreased absorption of phosphorus and from interference with phosphorylation processes in the tissues.
Selected parameters of lipid metabolism (cholesterol, HDL cholesterol, LDL cholesterol, atherogenic index, triacylglycerols, vitamin C, vitamin E, vitamin E/cholesterol, plasma fatty acid profile) and pro-oxidative/anti-oxidative parameters (conjugated dienes of fatty acids, activity of catalase and glutathione peroxidase) were estimated in blood of 59 healthy vegetarians aged 19-30 years. When compared to non-vegetarians, no incidence of obesity, low levels of cholesterol, LDL cholesterol, atherogenic index or triacylglycerols, HDL cholesterol levels on the margin of 1.4 mmol/l (boundary level between standard and reduced risk) as well as a higher plasma content of polyunsaturated fatty acids and a higher 18:2/18:1 ratio were all favourable consequences of vegetarianism with respect to atherosclerosis prevention. These factors are completed by higher levels of protective compounds with antisclerotic activity (vitamin C, vitamin E/cholesterol – protecting LDL from lipoperoxidation) as well as by beneficial pro-oxidative/anti-oxidative parameters (low values of conjugated dienes, significantly higher activity of catalase, higher level of vitamin C).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.