Introduction: The prevalence of gallstones varies between the population in each country and the ethnicity of the country. Incidence is reported in 11-36% of the world's population, 10% in the population of Western countries. In developing countries the prevalence varies from 15-25%. Research on the analysis of the bile acid composition of the microbiota shows the role of bacteria in the formation of pigment stones. From several patients after cholecystectomy, infection and sepsis occurred, the patient's bile culture -bacteria were found. Methods: To analyze the type of gallstones macroscopically with culture to see the presence or absence of infection with microorganisms in bile. patients with cholelithiasis or gallstones at HAM Hospital and Network Hospital. Results: In this study several results were found, namely stones occur mostly at the age of over 40 years, occur more in women, cholesterol stones are the most stones, on examination of bacteria more are negative, in samples with germs found Klebsiella pneumoniae the most and Enterococcus faecalis the least, the number of cholesterol and pigment stones is the same in proportion for the age group under 40 years. it was found that there was a relationship (p<0.05) between the type of stone and the lab results. Conclusion: In this study, female patients generally had positive stones and males were more dominantly negative, and the type of stone had a relationship with the microorganism lab results.
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