Twill copper fabrics (3/1) were produced using a single cylinder handloom jacquard weaving machine. The effect of varying weft density, warp density, wire diameter, and lay-up angle on electromagnetic shielding effectiveness was studied. The electromagnetic shielding effectiveness (EMSE) of various copper woven fabrics was obtained using a coaxial transmission line holder in the frequency range of 144–3000 MHz. With an increase in the number of conductive fabric layers, warp density, and weft density, an increase in shielding effectiveness is observed. With an increase in wire diameter, a decrease in shielding effectiveness has been observed.