Abstract. This article summarizes the results of a study of the cultural properties of the swine reproductive and respiratory syndrome virus isolate in transferable cultures of MA-104, PK-15, MARC-145 and Vero cells. The purpose of our research was to determine the sensitivity of cell culture lines to the swine reproductive and respiratory syndrome virus isolate, which is necessary for obtaining a highly active antigen as the main component of diagnostic and vaccine biologics. An isolate of the virus was isolated from a Piglet in the LPH of the Moscow region of the Kolomenskoye district. The isolated isolate of an infectious disease of pigs by the method of molecular biological analysis is characterized in the established order for this pathogen. Novelty. The possibility of reproduction in MARC-145 cell culture has been demonstrated. Results. It was shown that during reproduction of the virus isolate in cell culture for 96 ± 6 hours at a dose of 0.1 TCD50 / cell infection, an antigen with high biological activity was obtained. Infectious activity of the virus on the MARC-145 culture averaged 5.51 ± 0.45 lg TCD50/cm3 after the first three passages (after adaptation). Real-time PCR confirmed the presence of the virus genome in the test samples. Detection of the virus antigen in an infected cell culture was determined by the manifestation of a specific glow in the indirect immunofluorescence (RNIF) reaction in the cell culture monolayer fixed with acetone. It was found that FITZ-conjugate of rabbit polyclonal antibodies to pig immunoglobulins detected antigen-containing cells due to intense illumination.
The article presents the official information on the epizootic situation of FMD type А in the Middle East: Saudi Arabia, Iran, Armenia and Turkey in the period 2005-2017. Analyzing the number of outbreaks of FMD in these countries, it was found that, despite strict preventive measures associated with regular vaccination of susceptible animals, the main cause of the disease - the appearance of new genetic lines of topotype Asia: A/Iran-05 and G-VII, antigenically different from the production of FMD virus strains used in the vaccine. In this region, phylogenetic drift among the population of FMD type a virus manifests itself not only in the appearance of new lines, but also new sublines within the same line. . This is due, first of all, to the tense political situation in the region, when, due to uncontrolled migration of people and animals, there is a close contact of FMD-susceptible domestic and wild animals of different species and the alternate transmission of the virus to them. Due to the existing danger of spreading the virus in the countries of Central Asia and the Caucasus it is necessary to conduct studies on the selection and training of new relevant in respect of production of epizootic strains of FMD virus type A for inclusion in the composition of foot and mouth disease vaccine.
The purpose of the study was to analyze the effectiveness of the adjuvant complex based on saponin, glycerin and chitosan in the experimental series of an inactivated vaccine against cattle necrobacteriosis. 4 groups of animals (30 in each group) were selected for the study, aged 6 to 24 months. The first (1st) group consisted of calves 6 months old, the second (2nd) group consisted of animals 12 months old and the third group – animals 24 months old. The control 4 (fourth) group (non-vaccinated animals) contained 10 animals of each age group – 6, 12 and 24 months old. The animals were vaccinated using a needle-free injector at a dose of 0.4 cm3 in two points at a distance of 7–10 cm (0.2 cm3 each) twice at intervals of 4-6 weeks. Revaccinated after 6 months, with a single dose of 0.4 cm3 (at two points, 0.2 cm2 each). It was found that the vaccine with the multicomponent adjuvant had low viscosity and hardening temperature, moderate reactivity, high stability during storage, prolonged immunity in vaccinated animals. The immunity in animals was detected 21 days after the first vaccine administration and lasted at least 18 months after the last immunization. The necrobacteriosis incidence in animals of the control non-vaccinated group was 70-80%. During double vaccination, the average level of postvaccinal agglutinating antibodies in serum of experimental groups of animals was 312.08±74.12 – 62.13±69, 07. Humoral response in vaccinated animals provided reliable protection against Fusobacterium necrophorum disease within two years (observation period). Absence of disease symptoms in vaccinated animals during 2 years testified to the high preventive efficiency of the vaccine. The results obtained confirm the validity of the application of multicomponent adjuvant in inactivated sorbed vaccine as a means of specific prevention of animal necrobacteriosis.
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