Interpreting variable laboratory measurements of Helicoverpa zea Boddie susceptibility to toxins from Bacillus thuringiensis Berliner (Bt) has been challenging due to a lack of clear evidence to document declining field control. Research that links laboratory measurements of susceptibility to survival on Bt crops is vital for accurate characterization and any subsequent response to the occurrence of an implied H. zea resistance event. In this study, H. zea survival and the resultant damage to plant fruiting structures of non-Bt, Bollgard II, and Bollgard III cottons from two insect colonies with differing levels of laboratory susceptibility to Bt toxins were evaluated in large field cages. Laboratory bioassays revealed resistance ratios of 2.04 and 622.14 between the two H. zea colonies for Dipel DF and Cry1Ac, respectively. Differences between the two H. zea colonies measured via bioassays with Bollgard II and Bollgard III cotton leaf tissue in the laboratory were not statistically discernable. However, there was 17.6% and 5.3% lower larval mortality in Bollgard II and Bollgard III for the feral relative to the laboratory colony of H. zea, respectively. Although H. zea larval numbers in cages infested with the laboratory susceptible colony did not differ between the two Bt cottons, there were fewer larvae per 25 plants in Bollgard III than in Bollgard II cotton in cages containing tolerant insects. Cages infested with tolerant H. zea moths had higher numbers of total larvae than those containing the laboratory susceptible colony in both Bollgard II and Bollgard III cottons. Bollgard II and Bollgard III cottons received 77.4% and 82.7% more total damage to total plant fruiting structures in cages infested with tolerant insects relative to those containing the laboratory susceptible colony. The damage inflicted to fruiting structures on Bollgard III cotton by a feral H. zea colony with decreased measurements of laboratory susceptibility to Dipel DF and Cry1Ac indicate that the addition of Vip3A to third generation Bt cottons may not provide sufficient control in situations where infestations levels are high.
One of the largest and rarest Bebb willow (Salix bebbiana) communities in the United States occurs at Hart Prairie, Arizona. Low recruitment of the willow over the past several decades has been linked to inadequate soil water content for seed germination and seedling establishment. We tested a hypothesis that a prescribed burn would reduce biomass of and evapotranspiration by herbaceous plants, thereby increasing soil water content. Three treatments (unburned control, early-growing season burned, late-growing season burned) were applied in year 2001 to replicated plots in fern-and grass-dominated herbaceous communities. Soil water content (0-30 cm) was measured weekly in plots during the 2001, 2002, and 2003 growing seasons. Both early-and late-season burning reduced herbaceous biomass in the fern-dominated community in 2002 and 2003 and reduced biomass in the grass-dominated community in 2002 but not in 2003. Soil water content increased for approximately four weeks in 2001 following the early-season burn, but the early-season and late-season burns reduced soil water content in both communities over much of the 2002 and 2003 growing seasons.Thus, early-season burning may benefit willow seed germination by increasing soil water content immediately following burning but be detrimental to germination in the second and third growing seasons after burning because of drier soil. Large temporal variation in the effect of prescribed burning on soil water content will complicate the use of fire as a restoration tool to manage soil water available for threatened plants such as Bebb willow and for recharge of groundwater.
[1] Hart Prairie, Arizona, has the largest Bebb willow (Salix bebbiana) community in the United States; however, greater than 95% of willows are older than 80 years and regeneration by seed is not occurring. This study examined the evapotranspiration of two herbaceous communities that dominate the Hart Prairie watershed: (1) a mixture of bracken fern (Pteridium aquilinum) and graminoids and (2) dominantly graminoids. Transpiration during premonsoon and postmonsoon dry periods of 2000, 2001, and 2002 was estimated for each community by the difference in volumetric soil-water content (0-30 cm soil depth) between replicated plots that were clipped of all vegetation and control, unclipped plots. Transpiration rates estimated under conditions of minimal soil drainage varied between 0.63 and 2.4 mm/d for the fern-graminoid community and 0.57 and 1.1 mm/d for the graminoid community over the study. The fern-graminoid community produced more biomass than the graminoid community in all years, but generally had lower transpiration rates. Severe drought in year 2002 reduced growth and transpiration of the fern-graminoid community more than the graminoid community. Evaporation rates were estimated by temporal changes in soil-water content in clipped plots during dry periods, and were 54 to 474% of transpiration rates estimated under conditions of minimal soil drainage because of the dry and windy conditions that occur at the study site. Based on this study and a study of transpiration of scattered trees invading the meadow, transpiration by the herbaceous understory was higher than transpiration by trees during similar seasons.
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