A study on the RAPD profile of some pitcher plants (Nepenthes spp.) of Baturraden Botanical Garden collection was carried out. Seven individuals of particular concern were Mount Slamet semi-endemic and Java endemic species, i.e. N. adrianii and N. gymnampora. Both species of pitcher plants were taken from Baturraden Botanical Garden collection. Samples were then subject to genomic DNA extraction, after which RAPD markers were amplified using PCR technique with ten random primers, i.e. ES10G23, ES10A26, ES10C24, ES10G33, OPA-2, OPA-9, OPA-13, OPB-3, OPB-5 and OPB-7. The results show that the 10 primers produce 43 (67.2%) polymorphic bands and 21 (32.8%) monomorphic bands with size ranging from 130 to 1,500 bp. OPA-2 and OPB-3 produce highest polymorphism, while ES10G23 and OPA-13 produce most monomorphic bands. Dendogram constructed using UPGMA in MEGA 6.06 shows that at a genetic distance of 18% two clusters are formed. Both clusters are separated seemingly by geographic location. Key words: pitcher plants, RAPD, Baturraden Botanical Garden AbstrakTelah dilakukan penelitian tentang profil RAPD beberapa tanaman kantung semar (Nepenthes spp.) koleksi Kebun Raya Baturraden.Tujuh individu yang dipelajari merupakan spesies semiendemik Gunung Slamet dan endemik Jawa, yaitu N. adrianii dan N. gymnampora. Sampel kedua spesies kantung semar ini diambil dari koleksi Kebun Raya Baturraden. DNA genomik sampel diekstraksi untuk kemudian digunakan sebagai templat dalam amplifikasi marka RAPD menggunakan teknik PCR dengan 10 primer acak, yaitu ES10G23, ES10A26, ES10C24, ES10G33, OPA-2, OPA-9, OPA-13, OPB-3, OPB-5, dan OPB-7. Hasil penelitian menunjukkan bahwa ke-10 primer tersebut menghasilkan 43 (67,2%) pita polimorfikdan 21 (32,8%) pita monomorfikdengan ukuran berkisar dari 130 hingga 1.500 bp. OPA-2 and OPB-3 menghasilkan polimorfisme tertinggi, sedangkan ES10G23 dan OPA-13 menghasilkan pita monomorfik terbanyak. Dendogram yang dikonstruksi menggunakan UPGMAdalam MEGA 6.06 memperlihatkan bahwa dua klaster terbentuk pada jarak genetik 18%. Kedua klaster tersebut cenderung dipisahkan oleh lokasi geografi.
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