Enterobacter sp. MR1 an endophytic plant growth promoting bacterium was isolated from the roots of Butea monosperma, a drought tolerant plant. Genome sequencing of Enterobacter spp. MR1 was carried out in Ion Torrent (PGM), Next Generation Sequencer. The data obtained revealed 640 contigs with genome size of 4.58 Mb and G+C content of 52.8 %. This bacterium may contain genes responsible for inducing drought tolerance in plant, including genes for phosphate solubilization, growth hormones and other useful genes for plant growth.
Pseudomonas spp. MR3 was isolated from the surrounding soil of pesticide manufacturing industries of Ankleshwar, Gujarat. Under laboratory conditions these microbes were able to degrade up to 500 ppm of methyl parathion within 72 h. Genome sequencing of Pseudomonas spp. MR3 was carried out inIon Torrent (PGM), next generation sequencer. The data obtained revealed 1,268 contigs with genome size of 2.99 Mb and G ? C content of 60.9 %. The draft genome sequence of strain MR3 will be helpful in studying the genetic pathways involved in the degradation of several pesticides.
Sustainable production of ethanol as alternative renewable fuels, there is a need for a yeast strain which can convert the non-food substrate into ethanol economically. Extensive screening program for such yeast for bioethanol production was attempted from the diverge ecosystem. That resulted into isolation of a yeast strain, designated strain ETDLT1, which was capable of ethanol fermentation using lactose as carbon source even at elevated temperatures. Beside this the strain is ethanol tolerant up to 12 %. The lactose fermentation performance of selected ETDLT1 strain was evaluated. It was found to produce 28 g/l of ethanol with yield of 0.36 %. (p/s, g/g). In laboratory fermentation ETDLT1 strain found significant potential for its ability for bioconversion of dairy processing wastes rich in lactose into ethanol in single step fermentation. Therefore, it was further selected for thorough characterization. Results of characteristics confirm the ETDLT1 isolate as the strain of Kluyveromyces marxianus.
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