inspection of the TC. Furthermore the activator Mgions 31 may reach easily the sites of 5-nucleotidase or alk. phosphatase activity in the nucleus, resp. cytoplasm 32 . The decrease in ac. phosphatase values with salt-containing SM is not in contradiction with the aforesaid. In presence of salts may be a tendency of chelating, which may inhibit the acid phosphatase 31 ' 50 ' 54 ; further explanation may be that the morphological integrity of the organelles is preserved in presence of small amount of inorganic ions 32,36 . A matter of fact the vital staining properties of the cells with Iso II + salts (exp. of Fig. 6) were better, than those of the parallel TCs with SM Iso II alone. Thus the "unmasking" of this lysosomal biocatalysts 33 in absence of salts may account for the higher activities with the less "complete" substrate-medium.The purpose of this publication was to give the description of the techniques and the underlaying ideas, illustrated by pertinent examples. More details and the adaption of various other enzyme-methods will be reported separately, together with further work on special problems 9 ' 41 .
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