Phencyclidine and four analogues were tested for their capacity to inhibit total protein synthesis in a brain homogenate. At 1.0 mM they were all found to be potent inhibitors with values ranging from 36% to 96% inhibition. At this high concentration two of the analogues were equal to or more effective than the classic protein synthesis inhibitors cycloheximide and emetine. At lower concentrations the inhibition dropped off sharply to 18% at 1.0 X 10(-4) M and 9% at 1.0 X 10(-5) M for phencyclidine. If the inhibition observed in the brain homogenate occurs in vivo it may account for the high incidence of memory loss reported with phencyclidine use.
Anumber of lysine-auxotrophic mutants of Pseudomonas aeruginosa P A 0 1 were isolated through mutagenesis by means of N-methyl-N-nitrosoguanidin (MACH et al., unpublished). Using the cross feeding test and growth tests classification of lysine mutants was not possible. The investigation of diaminopimelic acid decarboxylase (DAP-DC) showed, that none of these mutants had a n active enzyme, except for the mutants with a high number of revertants. The appearance of only one mutant type is attributed to the insufficient availability of DAP.
A number of lysine-auxotrophic mutants of Pseudomonas aeruginosa PAO1 were isolated through mutagenesis by means of N-methyl-N-nitrosoguaniine (Mach et al., unpublished). Using the cross feeding test and growth tests classification of lysine mutants was not possible. The investigation of diaminopimelic acid decarboxylase (DAP-DC) showed, that none of these mutants had an active enzyme, except for the mutants with a high number of revertants. The appearance of only one mutant type is attributed to the insufficient availability of DAP.
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