chain at C-17, is almost as effective an inhibitor. The effect of substitution at the 11-position depends upon the nature of the oxygen function. The presence of a ketone group at C-ll, as in cortisone-21-succinate, does not decrease inhibitory activity as much as does a C,G1--OH group, such as is present in hydrocortisone-21-succinate. Alteration in the A ring, either by formation of a derivative at C-3 [testosterone-3-O-( carboxymethyl)oxime] or by aromatization of the ring (estrone and estradiol), greatly decreases or abolishes inhibitory activity. It would appear, from these results, that the structural features of ring A, that portion of the hapten molecule which is furthest removed from the point of attachment to the BSA, determines, to a great extent, the specificity of the reaction between T-17-BSA and anti-T-17-BSA. It is expected that continuation of studies of this type, by use of additional soluble steroid derivatives, will reveal more precisely the chemical basis of specificity.An interesting example of specificity is depicted in Fig. 1 , in which the inhibiting effectiveness of T-17-succinate and C-21-succinate on the reactions of C-21-BSA and T-17-BSA with an antiserum toC-21-BSA are plotted. C-21-succinate, the homologous hapten, equally inhibits the reaction with the homologous antigen, C-21-BSA, and the heterologous antigen, T-17-BSA. On the other hand, the heterologous hapten, T-17-succinate, inhibits the cross-reaction with T-17-BSA much better than it inhibits the reaction with the homologous antigen, C-21-BSA. Thus, although anti-C-21-BSA cross-reacts with T-17-BSA, by addition of a suitable concentration of T-17-succinate it should be possible to inhibit practically completely the cross-reaction with negligible effect on the homologous reaction. It is hoped that, in this manner, the in vivo activity of anti-C-21-BSA may be studied without complicating cross-reactions, and such investigation, are now in progress.It has thus been demonstrated that several steroid hormones may act as haptens when coupled to a protein, and antibodies with steroid specificity may be elicited (5). References and Notes chain at C-17, is almost as effective an inhibitor. The effect of substitution at the 11-position depends upon the nature of the oxygen function. The presence of a ketone group at C-ll, as in cortisone-21-succinate, does not decrease inhibitory activity as much as does a C,G1--OH group, such as is present in hydrocortisone-21-succinate. Alteration in the A ring, either by formation of a derivative at C-3 [testosterone-3-O-( carboxymethyl)oxime] or by aromatization of the ring (estrone and estradiol), greatly decreases or abolishes inhibitory activity.It would appear, from these results, that the structural features of ring A, that portion of the hapten molecule which is furthest removed from the point of attachment to the BSA, determines, to a great extent, the specificity of the reaction between T-17-BSA and anti-T-17-BSA. It is expected that continuation of studies of this type, by use of addition...
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