The thermoelectrogravitational electrophoresis column without reservoirs has been conceived following the principle of the Clusius-Dickel thermal diffusion column. The transport equation approach in thermal diffusion developed by Jones and Furry to explain the behavior of a conventional thermogravitational thermal diffusion column has been applied to describe the electrophoretic separation in the thermoelectrogravitational electrophoresis column. Equations have been established for a system in which one single or one hypothetically single component is mobile and the other species are at their isoelectric points.Theoretical calculations for the velocity profiles, temperature distribution, and the steady state batch separation factors have been made with field strength, temperature difference, membrane spacing, and electric mobility as variables, and expected trends of the results are discussed using the steady state solution of the transport equation for batch operation.
SCOPEThe objective of these studies was the experimental definition of a mathematical theory for a continuous thermoelectrogravitational liquid diffusion column without reservoirs in order to provide guidelines for the design of separation systems using these diffusional fluxes. The work is an extension of analysis for identical geometry for the thermal diffusion column without reservoirs.
CONCLUSIONS AND SIGNIFICANCEExperimental results for the separation of bovine albumin from buffer showed that the & e o v gave qualitative predictions of the influence of design variables on separation and capacity consistent with previous results for liquid thermal diffusion columns of the same type. The preliminary theory suggests that moderate laboratory data will suffice for the design of larger scale continuous separations using both thermal and electro diffusion with convection.The purpose of this work was to develop suitable mathematical theory and to explore the possibility of constructing thermoelectrogravitational electrophoresis columns without reservoirs and to obtain sufficient experimental data to establish the theory. In the present paper, a basic mathematical theory for theoretical analysis of such columns is developed. Two subsequent papers will be devoted to the presentation of further theoretical development and experimental work and a discussion of the results obtained. A summary of previously reported experimental apparatus is presented in Prabhudesai ( 1965), and 0. K. Crosser is at the University of Missouri, Rolla, Missouri. J. E.
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