No abstract

Zebra mussel (Dreissena polymorpha) is considered as one of the 100 most harmful IAS in the world. Traditional detection methods have limitations, and PCR based environmental DNA detection has provided interesting results for early warning. However, in the last years, the development of isothermal amplification methods has received increasing attention. Among them, loop-mediated isothermal amplification (LAMP) has several advantages, including its higher tolerance to the presence of inhibitors and the possibility of naked-eye detection, which enables and simplifies its potential use in decentralized settings. In the current study, a real-time LAMP (qLAMP) method for the detection of Dreissena polymorpha was developed and tested with samples from the Guadalquivir River basin, together with two real-time PCR (qPCR) methods using different detection chemistries, targeting a specific region of the mitochondrial gene cytochrome C oxidase subunit I. All three developed approaches were evaluated regarding specificity, sensitivity and time required for detection. Regarding sensitivity, both qPCR approaches were more sensitive than qLAMP by one order of magnitude, however the qLAMP method proved to be as specific and much faster being performed in just 9 min versus 23 and 29 min for the qPCR methods based on hydrolysis probe and intercalating dye respectively.

Erosión and sediment yield are a significant problem in the Guadalquivir River basin. Such phenomena are largely driven by a land use devoted to intensive cultivation of olive trees, with a large socioeconomic influence in Andalusia. This sediment overload in rivers causes serious impacts on all fluvial ecosystem components.In this study we assess the chronic effect of sediment yield on fish communities at 104 river sites located in two different sub-catchments -the Bembezar and Guadajoz rivers -both with different lithological composition and erosión rates. Sediment yield was estimated using a semi-quantitative Factorial Score Model (FSM), developed specifically for Spanish rivers. The fish populations of both basins were evaluated in composition and abundances by the study of Fernández-Delgado et al., 2014. The influence of sediment yield on the fish community was analyzed using General Additive Models.The sediment yield was higher in the Guadajoz basin (921 T/Km2 per year) than in Bembezar (701 T/Km2 per year). In the former, fish communities were poorer in both fish density and diversity, with Luciobarbus sclateri as the only substantially present species and a significant relationship between sediment yield and load, and fish density. In contrast, in the Bembezar basin, sediment yield was correlated with total fish density, including Luciobarbus sclateri, Pseudochondrostoma willkommii, Cobitis palúdica, ¡berochondrostoma lemmingii, Anaecypris hispánica, and Cyprinus carpió. Intermedíate valúes of sediment yield led to máximum densities, while those higher decreased the density of these species.

This study analyzes the somatic condition of southern Iberian barbel Luciobarbus sclateri (Günther, 1868) in the Guadiamar River (SW Iberian Peninsula). This river was seriously affected by a toxic spill of about 4 million cubic meters of acidic water and 2 million cubic meters of mud rich in heavy metals. Once the spill removal works concluded, sites affected and unaffected by the accident were sampled to study its effects on the fish fauna. The ecological variables registered were related to water quality, physical state of reaches, ecological quality, resources exploited by fish, and potential intra-specific interactions. From an initial fifteen ecological variables, seasonal water flow and pH explained most of the variation in barbel condition. This study shows that the Guadiamar River, fifty-six months after the accident, is still undergoing a recovery process where, 2 beyond ecological variables, proximity to the affected area is the most influential factor for fish condition.

The Guadiamar River (SW Iberian Peninsula) received a major toxic spill (6 hm 3 ) from a tailing pond in 1998 that defaunated 67 km of the main stem. Following early mud removal works, the fish assemblage was annually monitored at four affected sampling sites and one located in the upstream non-affected reach of the Guadiamar River as reference. Fish abundance and assemblage structure were analyzed. Principal response curve (PRC) was applied to assess the recovery trends and to identify the most influential species. A non-metric multidimensional scaling (NMDS) ordination and PERMANOVA were applied to evaluate changes in fish assemblage structure between sites and years. Overall, the affected reaches harboured fish within two years of the spill. Colonists arrived mainly from the upstream and downstream non-affected Guadiamar River reaches and, to a lesser extent, from three lateral tributaries. It is likely that the proximity, connectivity and environmental conditions of non-affected fish sources greatly influenced the recolonization process in each site. The structure of the fish community in the affected sites was initially similar to that in the unaffected reference stretch, but changed dramatically with time and each site followed its own trajectory. Currently, long-term threats such as mining leachates, urban sewage, agricultural pollution and exotic fish species expansion, have probably exceeded the initial spill effect. This highlights the large effect of anthropogenic factors on freshwater ecosystem resilience, and the need to significantly reduce both pollution and exotic species if the affected reach of the Guadiamar River is to recover fully.