The oxidation of indigo carmine by potassium hexacyanoferrate(III) is catalyzed by trace of silver nanoparticle (AgNP). The reaction is followed UV-Vis absorption spectrophotometrically by measuring the change in the absorption spectra (l max 612 nm). The catalytic oxidation reaction is used for the quantification of indigo carmine. The calibration graph was linear in the concentration range 50 nM-1.8 µM of indigo carmine. The variables affecting the method have been optimized. The method is applied to the determination of indigo carmine in groundwater samples with the satisfactory results.
A new kinetic method has been developed for the determination of iodine in water samples. The method is based on the catalytic effect of I(-) with the oxidation of Indigo Carmine (IC) by KBrO(3) in the sulfuric acid medium. The optimum conditions obtained are 0.16 M sulfuric acid, 1 × 10(-3) M of IC, 1 × 10(-2) M KBrO(3), reaction temperature of 35°C, and reaction time of 80 s at 612 nm. Under the optimized conditions, the method allowed the quantification of I(-) in a range of 12-375 ng/mL with a detection limit of 0.46 ng/mL. The method was applied to the determination of iodine in river and city water samples with the satisfactorily results.
A simple and sensitive kinetic-spectrophotometric method is developed for the determination of trace amounts of iodine in blood serum samples based on its catalytic effect on the oxidation of Nile Blue A by potassium bromate in sulfuric acid medium and at 25°C. The absorbance is measured at 595.5 nm with the fixed-time method. The optimization of the operating conditions regarding concentration of the reagents, temperature, and interferences are also investigated. The calibration curve is linear over the concentration range between 20.0 to 500.0 ng ml(-1) of iodine with good precision and accuracy. The detection limit of the method is down to 12.0 ng ml(-1). The relative standard deviation for a standard solution of 100.0 ng ml(-1) of iodine is 1.32% (n = 10). The proposed method provides a highly sensitive, selective, and relatively rapid assay for iodine at ultra trace level without any pre-concentration and separation step. The method was applied to the determination of iodine in blood serum samples. The analytical results of the real samples were in excellent agreement with standard method.
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