Zusammenfassung: Es wird ein Verfahren beHierzu werden die Cobalamine in der Vitamin-B 12 -schrieben, nach dem es möglich ist, die sehr emp-Coenzym-Synthetase-Reaktion adenosyliert und findliche Bestimmungsmethode für 5'-Desoxy-5'-das gebildete 5'-Desoxy-5'-adenosylcobalamin adenosylcobalamin durch die Propandiol-Dedurch die Propandiol-Dehydratase-Reaktion behydratase-Reaktion auf die Bestimmung von stimmt. Der dabei entstehende Aldehyd wird mit Cyano-und Hydroxocobalamin zu erweitern.Hilfe der Alkohol-Dehy drogenase-Reaktion erfaßt.
An Enzymic Assay for the Determination of Vitamin B 12Summary: A procedure which extends the very and the resulting 5'-deoxy-5'-adenosylcobalamine sensitive propanediol-dehydratase reaction for can be determined by the propanediol-dehydrat-5'-deoxy-5'-adenosylcobalamine determination ase reaction. Aldehyde, the product of the latter, to cyanocobalamine and hydroxocobalamine is can be registered by the alcohol dehydrogenase described. In this procedure, the cobalamines are reaction, adenosylated by vitamin B 12 coenzyme synthetase
Analyse beträgt in Lösungen von 1.5 mg/ml f 1.52 % ( s , , , n = 1 i), in Tabletten mit 1 mg/Dosis f 3.3 % (n = 9).
The Quantitation of Benzalkonium ChlorideBenzalkonium chloride is a mixture of homologous alkyl(benzy1)dimethylammonium chlorides. For the quantitative determination of benzalkonium chloride, the ratio of the homologues must be known. This can be easily determined by hplc on a cyano column and is the basis for the volumetric determination of the benzalkonium chloride content of a standard. On this standard is based the quantitative hplc analysis of pharmaceutical products, which is required to assess the stability ofthe active principle in liquid and solid preparations. The reproducibility of the method is I 1.52 % (Q, n = 1I)in solutions with a concentration of about 1.5 mg/ml and I 3.3 % (n = 9) in tablets with 1 mg/dose.
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