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The nitrogen dynamics of plants can be quantified using the variation in their δ 15 N level. This reveals details of plant physiological characteristics and the relationship between plants and their growth conditions. To better understand plant nitrogen dynamics and the effects of external temperature changes on their nitrogen isotopic composition, we investigated the δ 15 N characteristics in Triticum aestivum and its mother soils during the plant's life cycle. We found that under field conditions, the plant's leaves and roots δ 15 N significantly changed. The δ 15 N values in Triticum aestivum changed from -1.6‰ to -8.1‰ for leaves and from -2.0‰ to -8.8‰ for roots, respectively. δ15 N values for both, the leaves and roots were positively correlated with temperature. However, the foliar δ 15 N corresponded more strongly to air temperature, while the root δ 15 N corresponded to soil temperature. δ 15 N values of leaf and root both changed around 0.2‰ in response to a 1 degree change in temperature. Plant roots or shoot material cannot reflect the whole plant δ 15 N values due to a considerable difference between the δ 15 N values of root and leaf. However, the variations in leaf and root δ 15 N provide useful proxies to trace seasonal plant nitrogen cycles.