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This work introduces a new vision-based approach for estimating chlorophyll contents in a plant leaf using reflectance and transmittance as base parameters. Images of the top and underside of the leaf are captured. To estimate the base parameters (reflectance/transmittance), a novel optical arrangement is proposed. The chlorophyll content is then estimated by using linear regression where the inputs are the reflectance and transmittance of the leaf. Performance of the proposed method for chlorophyll content estimation was compared with a spectrophotometer and a Soil Plant Analysis Development (SPAD) meter. Chlorophyll content estimation was realized for Lactuca sativa L., Azadirachta indica, Canavalia ensiforme, and Lycopersicon esculentum. Experimental results showed that—in terms of accuracy and processing speed—the proposed algorithm outperformed many of the previous vision-based approach methods that have used SPAD as a reference device. On the other hand, the accuracy reached is 91% for crops such as Azadirachta indica, where the chlorophyll value was obtained using the spectrophotometer. Additionally, it was possible to achieve an estimation of the chlorophyll content in the leaf every 200 ms with a low-cost camera and a simple optical arrangement. This non-destructive method increased accuracy in the chlorophyll content estimation by using an optical arrangement that yielded both the reflectance and transmittance information, while the required hardware is cheap.

We examine the transmission characteristics of a NOLM device using a symmetrical coupler, highly twisted fiber, and a quarter-wave (QW) retarder plate introducing a polarization asymmetry in the loop. We demonstrate high dynamic range with controllable transmissivity, and good stability over long times. We experimentally study the transmission behavior for different input polarization states and distinguish between different polarization components of the output beam. Experiments are in good agreement with our theoretical approach previously published. Appropriate choice of the input and output polarizations allows a very high dynamic range. The adjustment of the QW retarder and input polarization enables tuning the critical power over a wide range.

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