Insgesamt 28 Diole des Typs (II) und 61 Benzodioxincarbonsäuren(‐säureester) des Typs (IV) wurden auf verschiedenen Wegen dargestellt, darunter RU 24728 (IVa) (trans bezüglich der Gruppen ‐Ph und ‐COOH), RU 25247 (IVb) (trans) und 25961 (IVd) (trans), und auf ihre hypolipämische Aktivität untersucht.
Es wurde eine Anzahl Thiophene vom Typ (I)‐(IV) dargestellt, von denen der größte Teil durch Aroylierung der entsprechenden Thienylessigsäuren nach Friede]‐ Crafts gewonnen wurde.
Adult rat hepatocytes were cultured for 15 days on type I collagen-coated permeable membranes in a hormonally defined Waxman's modified medium supplemented with very low concentrations of insulin, glucagon and dexamethasone. Phase contrast examination showed that 15-day-old cultures still formed a regular monolayer of polygonal cells. In similarly aged cultures, intracellular glycogen was abundant and evenly distributed, while steatosis remained very limited. Scanning and transmission electron microscopy showed that well developed bile canaliculi could be observed on the lateral side of the hepatocyte membrane after 4 days of incubation and persisted for 2 weeks. These canalicular structures probably originated from coalescence of membrane invaginations observed in 1-day-old cultures. Transmission electron microscopy showed that the ultrastructure of the cells was very close to that of normal rat hepatocytes in the intact liver. These results suggest that rat hepatocytes cultured under these experimental conditions are able to develop and maintain tissue-specific cytochemical and morphological properties for at least 15 days.
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