A critical step in the pre-mRNA splicing reaction is the stable binding of U2 snRNP to the branchpoint sequence (BPS) to form the A complex. The multimeric U2 snRNP protein complexes SF3a and SF3b are required for A complex assembly, but their specific roles in this process are not known. Saccharomyces cerevisiae homologs of all of the SF3a, but none of the SF3b, subunits have been identified. Here we report the isolation of a cDNA encoding the mammalian SF3b subunit SAP 145 and the identification of its probable yeast homolog (29% identity). This first indication that the homology between yeast and metazoan A complex proteins can be extended to SF3b adds strong new evidence that the mechanism of A complex assembly is highly conserved. To investigate this mechanism in the mammalian system we analyzed proteins that cross-link to 32p-site-specifically labeled pre-mRNA in the A complex. This analysis revealed that SAP 145, together with four other SF3a/SF3b subunits, UV cross-links to pre-mRNA in a 20-nucleotide region upstream of the BPS. Mutation of this region, which we have designated the anchoring site, has no apparent effect on U2 snRNP binding. In contrast, when a 2'0 methyl oligonucleotide complementary to the anchoring site is added to the spliceosome assembly reaction, A complex assembly and cross-linking of the SF3a/SF3b subunits are blocked. These data indicate that sequence-independent binding of the highly conserved SF3a/SF3b subunits upstream of the branch site is essential for anchoring U2 snRNP to pre-mRNA.[Key Words: pre-mRNA splicing; S. cerevisiae homolog; branchpoint sequence; snRNP protein complex; spliceosomal complex A; UV cross-links] Received August 25, 1995; revised version accepted November 17, 1995.During pre-mRNA splicing, a series of highly dynamic spliceosomal complexes assemble in the order E ~ A --~ B ~ C. Assembly of these complexes on pre-mRNA involves the functions of >50 distinct protein components as well as five small nuclear RNAs (U1, U2, U4, U5, and U6 snRNAs) (for review, see Moore et al. 1993; Newman 1994). One of the key steps in the spliceosome assembly pathway is the formation of an essential basepairing interaction between U2 snRNA and the branchpoint sequence (BPS). This interaction is established in the A complex and is thought to position the branch-site adenosine for nucleophilic attack on the 5' splice site during catalytic step I of the splicing reaction ). The BPS is highly conserved in yeast (consensus, UACUAAC), but only weakly conserved in metazoans (consensus, YNRAY). Given the short length of the BPS, and its degeneracy in metazoans, it is likely that spliceosomal proteins function together with the U2 sn-RNA-BPS duplex to tether the snRNP to pre-mRNA during spliceosome assembly.In metazoans, affinity-purified A complex consists primarily of U2 snRNP-specific proteins; these include A' and B", as well as spliceosome-associated proteins (SAPs) 49, 61, 62, 114, 130, 145, and 155 (for review, see Hodges and Beggs 1994 ). SAPs 61, 62, and 114 correspond to s...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.