The virus associated with citrus ringspot (CRSV) was further characterized. Nucleic acids associated with the short and long filamentous particles were isolated and appeared to be single-stranded RNA. Nucleic acid preparations of crude extracts or partially purified preparations were not infectious when assayed on Chenopodium quinoa. A sedimentable double-stranded RNA was associated with CRSV infections in C. quinoa and citrus. An antiserum, prepared to CRSV purified by sucrose gradient centrifugation and agarose gel electrophoresis, was used to detect the virus by serologically specific electron microscopy (SSEM) and on nitrocellulose blots of agarose electrophoresis gels. Index words. citrus psorosis.
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