Materials exhibiting gas permeable porosity are of particular interest for a number of functional applications. Here, the authors show a new nanoporous superalloy membrane, far surpassing existing gas permeable porous metals in fineness and regularity of the porosity. This may not only open the path for new medical applications it may also help realizing the vision of miniaturized fuel cells powering laptops and cellular phones.
A fragment of the gp‐36 gene of the Human Immunodeficiency Virus type 2 (HIV‐2) was fused to a stabilizer sequence, which encodes for the first N‐terminal 58 amino acids of the human interleukin‐2. The fused protein was expressed under the control of the tryptophan promoter in Escherichia coli, and expressed as 20% of the total cellular protein. Transmission electron microscopy indicated that the fusion protein formed cytoplasmic insoluble inclusion bodies. Inclusion bodies were semipurified by a wash pellet cell procedure, rendering a material with a purity higher than 70% by SDS–polyacrylamide gel electrophoresis. After solubilization with urea, this preparation was further purified by gel‐filtration chromatography up to 95% purity.
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