Plants of the partial amphiploid Inia 66/Thinopyrum distichum (2n = 70)//Inia 66 (2n = 56) were used as male parents in crosses with the monosomic series in the common wheat cultivar Inia 66. The genome and homoeologous group of the monosomic used in the cross affected the distribution of chromosome number of the progeny plants in the F2 and F4. Meiosis in the pollen mother cells of the B1F7 partial amphiploids was not stable, and not different from that of the B1F1 in which univalents and multivalents were observed. Disomic addition lines were selected on the basis of morphology and meiotic stability in the F2, F4 and F5. Eleven of the fourteen possible wheat-Th. distichum disomic addition lines were identified using chromosome C-band pattern, as well as size and arm ratio, as genetic markers. Addition of T. distichum chromosome J (dll) produced a phenotype indicating homoeology with wheat group-2 chromosomes. Clear indications of homoeology based on morphological characteristics were not obtained in any of the other addition lines, probably due to the mixed homoeology of the Th. distichum chromosomes relative to wheat. The addition lines were all susceptible to leaf rust, unlike the germplasm-line Indis which carries a leaf rust resistance gene on a translocation segment derived from Th. distichum. Instability of meiotic pairing was observed in all addition lines. The stability, or not, of progeny chromosome counts did not reflect the level of chromosome pairing instability in the parental plants. SDS-PAGE for gliadin-type seed proteins revealed two addition lines which expressed seed storage proteins uncommon to Inia 66 but typical of Th. distichum.
Thinopyrum distichum is indigenous to the southern and south western coastal shores of South Africa. Like many of the Thinopyrum species it can be hybridized with wheat. The resulting progeny treated with colchicine produce fertile amphiploids. The need to distinguish the Th. Distichum chromosomes from one another and from those of wheat prompted the investigation of the C-band distribution. The chromosome pairs of Th. distichum were distiguishable from each other and from those of wheat using C-band patterns, morphology and size as identification criteria. The chromosomes ranged from heterobrachial to metacentric with interstitial and telomeric C-bands. The C-band patterns of Th. distichum were similar, but not identical, to those of other Thinopyrum species.
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