Applied participatory research on large-ruminant health and production was conducted in six villages in northern Lao PDR. Three villages were classified as ‘high intervention’ (HI) and the remaining three as ‘low intervention’ (LI) sites, with a suite of health and productivity interventions implemented in the HI sites enabling comparison of outcomes with the LI sites, where only a vaccination program was introduced. A 3-year longitudinal study to establish baseline production variables, including liveweight, average daily weight gain (ADG) and reproductive performance, was conducted. The study involved 1500 head of cattle and buffalo that were ear-tagged and weighed every 3–4 months between 2008 and 2011, producing 10 data-collection points. Significant differences in ADG of the cattle between the provinces (P < 0.001), but not between HI and LI villages (P = 0.39), was observed. Low calving rates (51–75% and 41–52%) and inter-calving intervals (13.6–15.7 and 18.6–20.6 months) for cattle and buffalo, respectively, were observed. An on-farm large ruminant-fattening trial (n = 44) was conducted over a 4-month period to examine differences in productivity between cut-and-carry stall fattening (n = 26) and free-grazing (n = 18) systems. Cattle and buffalo in fattening stalls (320 and 217 g/day) had significantly greater ADG than those free-grazing (40 and 85 g/day) (P < 0.001 and P = 0.001). The increase in sale value for fattened cattle and buffalo was US$78 and US$123, respectively. The longitudinal study indicated that if risks of important diseases such as foot and mouth disease and haemorrhagic septicaemia are controlled by vaccination and biosecurity, improved productivity outcomes in northern Lao PDR can be achieved by establishing forage plantations to better manage variations in seasonal availability of feed and enabling fattening. We conclude that improved large-ruminant productivity, by improving health and nutrition practices, offers opportunities for smallholder farmers to increase livestock income, alleviate rural poverty and improve regional food security in South-east Asia.

The buffalo is an important livestock resource in several countries of South Asia and the Mediterranean regions. However, reproductive efficiency is compromised due to known problems of biological and management origins, such as lack of animal selection and poor nutrition. Under optimal conditions puberty is attained at 15 to 18 months in river buffalo, 21 to 24 months in swamp buffalo and is influenced by genotype, nutrition, management and climate. However, under field conditions these values deteriorate up to a significant extant. To improve reproductive efficiency, several protocols of oestrus and ovulation synchronization have been adopted from their use in commercial cattle production. These protocols yield encouraging pregnancy rates of (30% to 50%), which are comparable to those achieved in buffaloes bred at natural oestrus. The use of sexed semen in buffalo heifers also showed promising pregnancy rates (50%) when compared with conventional non-sexed semen. Assisted reproductive technologies have been transferred and adapted to buffalo but the efficiency of these technologies are low. However, these latest technologies offer the opportunity to accelerate the genetic gain in the buffalo industry after improving the technology and reducing its cost. Most buffaloes are kept under the small holder farming system in developing countries. Hence, future research should focus on simple, adoptable and impact- oriented approaches which identify the factors determining low fertility and oestrus behaviour in this species. Furthermore, role of kisspeptin needs to be explored in buffalo.

A retrospective investigation of financial losses incurred by large ruminant smallholder farmers due to outbreaks of foot and mouth disease (FMD) in 2010-12 in northern Laos was conducted in 2012. The aim was to support recommendations on sustainable transboundary animal disease control strategies in the Greater Mekong Subregion (GMS). Large ruminant smallholders in the three northern provinces of Luang Prabang (LPB), Xiengkhoung (XK) and Xayyabouli (XYL) were interviewed (n = 310). Financial losses were determined, including direct losses due to mortality (100% of pre-FMD sale value) and morbidity (difference between the expected sale price pre-FMD and 1 month following onset of FMD), and indirect losses due to costs of treatments. The losses due to FMD per household varied between provinces (P < 0.001) and were USD 1124, USD 862 and USD 381 in LPB, XK and XYL, respectively, being 60, 40 and 16% of annual household income. Comparison of the costs of FMD with annual household income from sales of large ruminants indicated losses of 213, 181 and 60% of the income in LPB, XK and XYL, respectively. The variation in losses between provinces was due to differences in levels of morbidity with highest in LPB, treatment methods with antibiotic use common in LPB, age of animals sold and sale prices with higher prices in XK. Partial budget analysis of biannual FMD vaccination indicated an average net benefit of USD 22 and USD 33 for cattle and buffalo, respectively. However, vaccination alone is unlikely to control FMD in the region. Promotion of multiple large ruminant health and production intervention programmes to stimulate interest in biosecurity in addition to vaccination is recommended, providing a more sustainable pathway for poverty reduction through the current expansion of livestock investments in the GMS.

The Asiatic swamp buffalo (Bubalus bubalis) is an important livestock species in the Lao People's Democratic Republic (Laos), with smallholder farmers owning the majority of an estimated 774,200 buffalo. Of the estimated 226,400 farm households with buffalo, 78% have a herd size of four or fewer, kept as a storage of wealth, sale for beef, for manure as fertilizer, and decreasingly, draught power. The total meat consumption in Laos and China was approximately 21 kg and 58 kg per capita in 2009, with an annual growth rate forecast of 4.5% and 3.1%. Increasing demand for meat in both domestic and neighboring markets offers opportunities for smallholders to gain more income from their livestock, particularly buffalo. However improving buffalo production requires numerous management constraints to be addressed, including: health issues (parasites and endemic diseases, particularly Toxocara vitulorum and Haemorrhagic Septicaemia control), biosecurity and transboundary disease (Foot and Mouth Disease control), nutritional deficits, low reproductive performance, high slaughter rates of pregnant cows, undeveloped trade and marketing systems, limited veterinary and extension service capacity, and potentially climate change and policy impacts. This paper reviews available information on these constraints to identify remaining gaps in knowledge and offer potentially suitable strategic interventions aimed at increasing the supply of buffalo in Laos. With the current rapid rate of economic development expected to continue in Laos and the Greater Mekong Subregion, expansion of the domestic and regional markets for food requires a more biosecure and sustainable supply of safe meat. Improving livestock health, productivity, processing and marketing, particularly of buffalo, is critical to ensuring poor smallholder communities can participate in and access these growing markets, providing improved rural livelihoods, poverty reduction and increased food security.

Transboundary animal diseases including foot-and-mouth disease and haemorrhagic septicaemia remain a major constraint for improving smallholder large ruminant productivity in the Mekong region, producing negative impacts on rural livelihoods and compromising efforts to reduce poverty and food insecurity. The traditional husbandry practices of smallholders largely exclude preventive health measures, increasing risks of disease transmission. Although significant efforts have been made to understand the social aspects of change development in agricultural production, attention to improving the adoption of biosecurity has been limited. This study reviews smallholder biosecurity risk factors identified in the peer-reviewed literature and from field research observations conducted in Cambodia and Laos during 2006-2013, considering these in the context of a change management perspective aimed at improving adoption of biosecurity measures. Motivation for change, resistance to change, knowledge management, cultural dimensions, systems theory and leadership are discussed. Due to geographical, physical and resource variability, the implementation of biosecurity interventions suitable for smallholders is not a 'one size fits all'. Smallholders should be educated in biosecurity principles and empowered to make personal decisions rather than adopt prescribed pre-defined interventions. Biosecurity interventions should be aligned with smallholder farmer motivations, preferably offering clear short-term risk management benefits that elicit interest from smallholders. Linking biosecurity and disease control with improved livestock productivity provides opportunities for sustainable improvements in livelihoods. Participatory research and extension that improves farmer knowledge and practices offers a pathway to elicit sustainable broad-scale social change. However, examples of successes need to be communicated both at the 'evidence-based level' to influence regional policy development and at the village or commune level, with 'champion farmers' and 'cross-visits' used to lead local change. The adoption of applied change management principles to improving regional biosecurity may assist current efforts to control and eradicate transboundary diseases in the Mekong region.