Objectives: Trypanosoma cruzi, the causal agent of Chagas disease in humans, is a widely spread protozoan in Latin America. Chronically infected people are asymptomatic during an indeterminate stage but can represent a significant risk of transmission due to blood donations and organ transplants. Blood transfusion is recognized as the second most important path for transmitting of Chagas disease; in addition, it can be recognized as the main important route in industrialized non-endemic countries. The aim of the present work was to detect anti-T. cruzi antibodies in blood donors in Cancun,