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Chlorinated secondary-treated effluent was used to irrigate a grassed 4-acre site at rates of 2 and 4 inches per week for periods of 11 and 14 weeks, respectively. Part of the site was drained by tile lines 5 feet below land surface. Chemical and bacteriological changes in the acidic ground water in the shallow sand aquifer and in the effluent from the drains were studied. Prior to irrigation, total coliform bacteria were not detected in ground water at the site. After irrigation, total and fecal colifonus were detected in the ground water at the site and downgradient. The nitrifying bacteria Nitrosomonas and Nitrobacter at the irrigated site were most abundant at the soil surface; their numbers decreased with depth.

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