Tubes of tomato juice agar were inoculated with Clostridium sporogenes and an Aspergillus sp. and incubated aerobically. The clostridium only grew when the Aspergillus was present. pH and oxygen concentration were measured through the depths of the tubes. The Aspergillus grew as a mat on the surface of the gel, removing oxygen and causing a local rise in pH. It was thought that the production of a microenvironment adjacent to the mycelial mat, consisting of raised pH and decreased oxygen concentration, enabled the clostridium to germinate and grow.
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